FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424864
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATAGA62030.4353397938329553No Hit
GTCTATTACTGTGCGAAAGGCCGGTGGGAACTAGTAGATGCTTGTGATAT51210.35940272194398903No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40970.28753621398252743No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39020.27385069732971007No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT38960.2734296045096234No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32730.22970613335728884No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32090.22521447660969746No Hit
GGGTATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGTCTCCTGATCTATGCTGCATC29180.20479147483549306No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA29060.2039492891953197No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG28180.19777326116738161No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26550.18633357288835986No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26490.18591248006827318No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG26390.18521065870146203No Hit
GTACTATGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATT26020.1826139196442608No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATATTCAT25910.18184191614076853No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24870.17454297392593257No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24460.1716655063220069No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23990.16836694589799447No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23330.1637349248770409No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC23230.16303310351022973No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23140.16240146428009972No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23060.1618400071866508No Hit
GATAAGTACTATGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCCAAGAACACGCTCTATCTG22830.16022581804298514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22230.15601488984211825No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22110.15517270420194487No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC22020.15454106497181486No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21740.15257596514474364No Hit
CTCCTGTGCAGCCTCTGGATTCAACTTCAATGAATATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG21690.15222505446133805No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCGCTCACCTT21600.15159341523120803No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT21160.14850540121723899No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT20580.14443483728973433No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20330.14268028387270643No Hit
GGCTAACAGTTTCCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT20310.1425399195993442No Hit
GGGCTGGAGTGGGTGGCAGTTACATCATATGATGGAACTGATAAGTACTA20200.14176791609585196No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT20090.1409959125923597No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20000.14036427336222967No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19990.14029409122554853No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19850.13931154131201293No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT19650.13790789857839064No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC19580.13741662362162285No Hit
GTACTTATCAGTTCCATCATATGATGTAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG19190.13467952029105934No Hit
GCATAGATCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC19180.13460933815437826No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19180.13460933815437826No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18980.13320569542075594No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18460.129556224313338No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18290.12836312798975902No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAA18130.12724021380286119No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18120.12717003166618007No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17640.12380128910548657No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA17250.12106418577492307No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17140.12029218227143082No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG16990.11923945022121409No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16970.11909908594785186No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16910.11867799312776518No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA16570.11629180048060726No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16550.11615143620724504No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGT16450.11544961484043391No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16440.11537943270375278No Hit
GCATAGTACTTATCAGTTCCATCATATGATGTAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACC16350.11474779347362275No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16120.11313360432995712No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA15940.11187032586969703No Hit
GCCTGAGACCTGAGGACACGGCTGTCTATTACTGTGCGAAAGGCCGGTGGGAACTAGTAGATGCTTGTGATAT15840.1111685045028859No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15660.10990522604262581No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15360.10779976194219237No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGAGCGGGAAA15230.10688739416533789No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15150.10632593707188896No Hit
GCTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTG14860.10429065510813665No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14630.10267646596447101No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14600.10246591955442766No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14520.10190446246097873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTA200.007263677751.9216241
TCTGGAG21650.041.90030370
GTGGTAT3550.040.7797781
GTATCAA20600.037.8150861
GTATATA655.491456E-737.1200521
TTCGGGG16650.032.6897970
TATCAAC23850.031.9327532
ACCTAGC553.268108E-431.34611313
ATCAACG24300.031.1995893
TCAACGC24450.030.7262884
CAACGCA25000.030.050315
GCGTGTT19300.029.8772951
ATTGTCT19550.029.7743264
AGCGTGT19550.029.66451650
TTGTCTC19800.029.28716565
AACGCAG25750.029.1781276
CGTGTTC19800.029.13930752
AATTGTC19900.029.0340163
TAGAGCG19800.028.92077847
AGAGCGT20000.028.81425748
Produced by FastQC (version 0.11.5)