FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628244
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25850.4114643355129535No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24370.3879066095338754No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23070.3672140123900905No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22900.3645080573789801No Hit
GTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCCC22640.3603695379502232No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22500.3581411044116617No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21000.3342650307842176No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20910.33283246636657093No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20340.3237595583881422No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19120.304340351837821No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18990.3022710921234425No Hit
GACCTAGGACGGTCACCCTGGTCCGTCCGCCGAAGACCACAGTACTGTCGCCTGTATAGGAACTGCAATAGTAGT18410.29303901032083074No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17760.28269271174893834No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16930.26948128434175256No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16650.26502441726462966No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16600.2642285481437149No Hit
GTTCCTATACAGGCGACAGTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTG16460.2620001146051534No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16360.2604083763633238No Hit
GACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTC16230.2583391166489453No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16150.2570657260554816No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15970.25420059722018834No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCGTCCGCCGAAGACCACAGTACTGTCGCCTGTATAGGAACTG15620.24862951337378472No Hit
GTATAGGAACTGCAATAGTAGTCAGCCTCGTCGTCAGCCTGGAGCCCAGA15420.24544603689012548No Hit
GTTATCGGCCTTCAGGGGTCTCCACTCGCTTCTCTGGCTCCAAGTCTGGC14510.23096121888947604No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14160.22539013504307243No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14020.22316170150451098No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13900.22125161561431547No Hit
CCACAGTACTGTCGCCTGTATAGGAACTGCAATAGTAGTCAGCCTCGTCG13730.2185456606032051No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13410.21345209822935038No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13030.2074034929103979No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12680.20183240906399424No Hit
CTTCAGGGGTCTCCACTCGCTTCTCTGGCTCCAAGTCTGGCAACGCGGCCTCCCTGACCATTTCTGGGCTCCAGG12380.1970571943385054No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12340.19642049904177358No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12210.1943512393273951No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11890.18925767695354034No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11650.1854375051731493No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11560.18400494075550264No Hit
CTATTGCAGTTCCTATACAGGCGACAGTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCC11470.18257237633785597No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11370.18098063809602638No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11350.18066229044766047No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCGTC11170.17779716161236717No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11150.17747881396400125No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11140.17731964013981827No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10990.17493203277707386No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10780.1715893824692317No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10720.17063433952413393No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10520.1674508630404747No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG10370.1650632556777303No Hit
GTCGATCACCATCCCCTGCATTGGAACCAGCAATGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAGCAACA10260.16331234361171773No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10050.15996969330387556No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9980.15885547653459484No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9890.1574229121169482No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9740.15503530475420377No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT9350.1488275256110683No Hit
TTTCTGGGCTCCAGGCTGACGACGAGGCTGACTACTATTGCAGTTCCTAT9300.1480316564901535No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9010.14341561558884763No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA8940.14230139881956694No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8720.13879957468754178No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8710.13864040086335883No Hit
CGACAGTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA8690.1383220532149929No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8660.13784453174244402No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8620.13720783644571216No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA8550.13609361967643147No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8550.13609361967643147No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC8430.13418353378623593No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8290.13195510024767446No Hit
GTACTGTCGCCTGTATAGGAACTGCAATAGTAGTCAGCCTCGTCGTCAGC8160.129885840533296No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8150.129726666709113No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG8050.12813492846728342No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA7930.12622484257708788No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7860.12511062580780716No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT7750.12335971374179458No Hit
CTCTTGGACAGTCGATCACCATCCCCTGCATTGGAACCAGCAATGACGTT7680.12224549697251387No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7640.12160880167578202No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG7600.12097210637905018No Hit
GGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAG7590.1208129325548672No Hit
GTGATCGACTGTCCAAGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCGTACTGGGCCCCGGATTCTGTGCC7550.12017623725813537No Hit
CTTATAACCACCAACGTCATTGCTGGTTCCAATGCAGGGGATGGTGATCG7520.1196987157855865No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCGTCCGCCGAAGAC7520.1196987157855865No Hit
CTGCAATAGTAGTCAGCCTCGTCGTCAGCCTGGAGCCCAGAAATGGTCAGGGAGGCCGCGTTGCCAGACTTGGAG7490.11922119431303763No Hit
CTCTCAGGGCATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGAATCCGGGGC7390.117629456071208No Hit
ACCTAGGACGGTCACCCTGGTCCGTCCGCCGAAGACCACAGTACTGTCGC7370.11731110842284208No Hit
GTTTGGGGACTTCGCCTGGGTGTTGCTGGTACCAGGAGACATACTTATAA7360.11715193459865911No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC7360.11715193459865911No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG7290.1160377178293784No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC7100.11301341516990215No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7080.11269506752153621No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA6990.11126250310388958No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG6960.11078498163134068No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6860.10919324338951109No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG6610.10521389778493707No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC6560.10441802866402226No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAG6540.10409968101565634No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT6500.1034629857189245No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCA6500.1034629857189245No Hit
ACAGTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT6490.10330381189474153No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT6460.10282629042219266No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6380.10155289982872895No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG6290.10012033541108231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGT308.913785E-657.72356844
TATTCCG252.5225367E-455.39241843
CGAATAC200.00672037452.94888364
CTGTCGA200.00724543951.95120644
CTCTATC200.007459089651.5707939
TATACAC3350.051.3142243
CTTATAC3500.049.1621
TGGTATC850.048.5372242
TTATACA3650.048.038552
GGTATCA4050.046.734251
AGGGGGG3350.046.41845370
GTGGTAT851.8189894E-1244.5349921
GTATCAA10500.042.9348141
CGTGAAA757.585186E-1041.98512657
TACACAT4100.041.9274755
ATACACA4200.041.7477874
CGCGTAT350.001337066639.49005539
ATCGCGT350.001343388839.4521837
TCGTATT459.8770026E-538.4207740
TATCAAC11950.037.4014172
Produced by FastQC (version 0.11.5)