FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628244
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53570.8526941761481208No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30080.478794863142346No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23730.37771948478616585No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23390.3723075747639452No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21630.34429298170774414No Hit
GTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCCC21470.3417462005208168No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21240.33808520256460867No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21010.33442420460840055No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20100.3199393866077511No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18720.29797339887050256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17960.28587618823259753No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17060.27155054405613105No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16800.26741202462737407No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16420.2613634193084216No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16250.25865746429731123No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16160.25722489987966457No Hit
GTTATCGGCCTTCAGGGGTCTCCACTCGCTTCTCTGGCTCCAAGTCTGGC15700.2499029039672484No Hit
GTTCCTATACAGGCGACAGTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTG15600.24831116572541878No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15520.2470377751319551No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15370.2446501677692107No Hit
GACCTAGGACGGTCACCCTGGTCCGTCCGCCGAAGACCACAGTACTGTCGCCTGTATAGGAACTGCAATAGTAGT15230.24242173423064925No Hit
GACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTC14590.23223460948293978No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCGTCCGCCGAAGACCACAGTACTGTCGCCTGTATAGGAACTG14190.22586765651562132No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14090.22427591827379173No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13670.21759061765810736No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13490.21472548882281406No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13220.21042779556987415No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12990.20676679761366604No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12760.2031057996574579No Hit
GTATAGGAACTGCAATAGTAGTCAGCCTCGTCGTCAGCCTGGAGCCCAGA12630.20103653994307946No Hit
CTTCAGGGGTCTCCACTCGCTTCTCTGGCTCCAAGTCTGGCAACGCGGCCTCCCTGACCATTTCTGGGCTCCAGG12600.20055901847053056No Hit
CCACAGTACTGTCGCCTGTATAGGAACTGCAATAGTAGTCAGCCTCGTCG12530.19944480170124984No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12530.19944480170124984No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12260.19514710844830988No Hit
GTCGATCACCATCCCCTGCATTGGAACCAGCAATGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAGCAACA11940.19005354607445515No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11750.1870292434149789No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11620.1849599837006004No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11460.18241320251367305No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11430.18193568104112415No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11190.17811550926073308No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11180.17795633543655012No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10990.17493203277707386No Hit
CTATTGCAGTTCCTATACAGGCGACAGTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCC10850.17270359923851242No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10540.16776921068884065No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10340.16458573420518144No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT10320.1642673865568155No Hit
TTTCTGGGCTCCAGGCTGACGACGAGGCTGACTACTATTGCAGTTCCTAT10250.16315316978753477No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9890.1574229121169482No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9750.15519447857838675No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9700.15439860945747194No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9620.15312521886400826No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9600.15280687121564232No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9550.15201100209472754No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG9370.14914587325943424No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA9360.14898669943525128No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT9350.1488275256110683No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9350.1488275256110683No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9230.1469174397208728No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCGTC9210.14659909207250685No Hit
GGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAG8920.141983051171201No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8670.13800370556662697No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8640.1375261840940781No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA8510.1354569243796996No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8240.13115923112675967No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8180.1302041881816619No Hit
CGACAGTACTGTGGTCTTCGGCGGACGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA8120.12924914523656414No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8010.12749823317055156No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAG7930.12622484257708788No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC7860.12511062580780716No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7800.12415558286270939No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG7720.12288219226924571No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA7690.12240467079669681No Hit
GTCTGGCAACGCGGCCTCCCTGACCATTTCTGGGCTCCAGGCTGACGACG7560.12033541108231834No Hit
CTCTTGGACAGTCGATCACCATCCCCTGCATTGGAACCAGCAATGACGTT7530.11985788960976945No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCGTCCGCCGAAGAC7500.11938036813722057No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7470.11890284666467169No Hit
CTCTCAGGGCATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGAATCCGGGGC7470.11890284666467169No Hit
GTGATCGACTGTCCAAGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCGTACTGGGCCCCGGATTCTGTGCC7340.11683358695029321No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG7290.1160377178293784No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC7270.11571937018101247No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC7250.11540102253264656No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA7250.11540102253264656No Hit
GTACTGTCGCCTGTATAGGAACTGCAATAGTAGTCAGCCTCGTCGTCAGC7140.113650110466634No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG7110.1131725889940851No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC7090.11285424134571918No Hit
CTGCAATAGTAGTCAGCCTCGTCGTCAGCCTGGAGCCCAGAAATGGTCAGGGAGGCCGCGTTGCCAGACTTGGAG7080.11269506752153621No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6910.1099891125104259No Hit
GTTTGGGGACTTCGCCTGGGTGTTGCTGGTACCAGGAGACATACTTATAA6850.10903406956532813No Hit
CTTATAACCACCAACGTCATTGCTGGTTCCAATGCAGGGGATGGTGATCG6720.10696480985094962No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT6470.10298546424637563No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG6450.10266711659800969No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGAATCCGGGGCCCAGTACGCCCTGACTCAGC6290.10012033541108231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATAA150.001990298771.9902567
AGTAGCG259.965183E-566.8343669
ACGAACC200.007425314751.6300230
CAATACT200.007467527451.556054
GGTATCA3450.050.8209951
GTATCAA9550.043.9186061
TAACACG350.001372450839.2808044
TATCAAC10750.038.6870232
TCGCCTA400.002632340934.40081828
ATCAACG12250.033.9498373
TCAACGC12350.033.674944
CAACGCA12500.033.5458075
AACGCAG12800.032.7595756
CGCAGAG13300.031.8016268
GGGTTTA3950.031.332681
AGAGTAC13400.031.3076811
CAGAGTA13400.031.3076810
TTATCAG553.3278257E-431.2460925
GTACGCT450.00462125630.66645236
TGACGTG450.004695784330.5663329
Produced by FastQC (version 0.11.5)