FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171609
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT4240.24707328869697978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG701.8189894E-1276.3036470
CGTATGC1000.048.5260541
CTCGTAT950.047.3896339
GCCGTCT1050.046.4477346
TCGTATG1050.046.188140
TGCCGTC1150.042.34615745
ATCTCCG1150.041.7662410
CGAGCCC1150.041.7662415
TCTCGTA1100.040.90334338
CGTCTTC1200.040.68992248
CCGTCTT1200.040.6658347
TATGCCG1200.040.5218843
ATCTCGT1200.040.33152437
CCCACGA1250.038.43614219
TCTCCGA1250.038.42494211
CTTATAC1350.038.153331
ATGCCGT1300.037.44899744
TATACAC1500.036.5951843
ACGAGAC1350.035.59939622
CACGAGA1350.035.5890221
Produced by FastQC (version 0.11.5)