FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171609
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8310.48424033704525987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC150.002415217568.5408628
TTAGCCC350.0014014839.09773613
TAGGGGT350.0014014839.0977364
TACGGGG2400.035.64634315
GTATCAA6250.035.5789381
GGTATCA3050.034.7713471
ACGGGTT400.002691762534.23046516
TACGGGT1004.110916E-1034.2204915
GTTGAGC400.00269947134.2105183
AGGTATC701.0331023E-634.2105182
ATCAACG6600.033.6921773
TATCAAC6700.033.189312
TCAACGC6650.032.9244084
CAACGCA6700.032.6787035
ACGGGGA1056.91216E-1032.60044516
AACGCAG6750.032.4366386
TAGTAGT450.004805904330.409354
AGTACGG5600.029.93420413
GTACGGG5750.029.74827614
GAGTACG5650.029.66929812
Produced by FastQC (version 0.11.5)