FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044600
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.043.152091
GTATCAA28250.039.9244231
TCAACGC33850.033.49444
CAACGCA34450.033.0110745
ATCAACG34800.032.77813
TATCAAC34800.032.679072
AACGCAG35050.032.445986
CTCCGAA800.004486363430.85237370
GCGTAGA1202.8806395E-530.85237370
ACGCAGA37600.030.2455227
AGAGTAC39050.029.21489511
CAGAGTA40300.028.39424910
CGCAGAG40600.028.0995468
GCAGAGT43000.026.5311999
AGTACGG34800.025.45371813
GAGTACG35200.025.2623912
GACTAAG1752.4374458E-1023.6409726
TGGTATC5400.022.9743752
GTGGTAT5500.022.576111
AGACTAA1854.8748916E-1022.3609425
Produced by FastQC (version 0.11.5)