FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044600
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10690.10233582232433468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29600.040.7756541
GGTATCA12150.036.22591
TATCAAC34200.034.8840182
ATCAACG34400.034.5812573
TCAACGC34450.034.5310634
CAACGCA35100.033.8916055
AACGCAG35350.033.4622046
ACGCAGA37800.031.3068457
GTGGTAT5700.030.7666951
CGGGTAT802.8311824E-630.09089717
AGAGTAC39400.030.03837811
CGCAGAG39550.029.9244548
CAGAGTA39900.029.5757310
GCAGAGT41000.028.6983229
TGGTATC5650.028.600432
ATCCCGT1354.8902264E-528.54967170
AGTACGG33200.027.25410513
ACGGGTA2400.027.22900816
GAGTACG33300.026.96562812
TACGGGT7850.024.98035615
Produced by FastQC (version 0.11.5)