FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879704
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33750.17954954609874746No Hit
GTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA32870.17486795793380236No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29500.15693960325668296No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA25130.1336912620284896No Hit
CAGTAATACAGAGCAGTGTCCTCACTTCGCAGGCTGCTCATCTGAAGAAA24330.1294352727876304No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGCGGCCCTGGT23710.1261368811259645No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGA22720.12087009444040125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21840.11618850627545614No Hit
CTCCTGCAGGGCTAGTGAGAGTATTAGCAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT19970.10624013142494775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19940.10608053182841552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19930.10602733196290479No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19830.1054953333077974No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19600.10427173640105036No Hit
GTATTACTGTGTGAAAGACCTCGGGTACGGCTCCCTCCTTCACTATTGGGGCCAGGGAACCCTGGTCACCGTCTC19360.10299493962879261No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCTTCGGAGGCCAGTTAGTAGTCTC19090.10155854326000263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32100.039.778581
GGTATCA13950.038.345121
TATCAAC38550.033.1036342
ATCAACG39300.032.471893
TCAACGC39600.032.0516934
CAACGCA39950.032.0298925
AACGCAG41450.030.9546
AGAGTAC42700.029.64792611
TCGTATG1103.570858E-828.3037540
CGCAGAG45300.028.0955858
GCAGAGT46300.027.488779
CAGAGTA47100.026.8782710
AGTACGG43800.026.2256513
GAGTACG44850.025.5347612
GTGTATA2500.024.83640113
TACGGGT3700.024.23908415
ACGCAGA53750.023.9989097
TTCGGGG11850.023.75343170
TATACAC3200.022.6343333
GTATATA1554.076719E-822.2649061
Produced by FastQC (version 0.11.5)