FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879704
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35090.18667832807718662No Hit
GTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA32680.17385716048909827No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28800.15321561267093117No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA24830.1320952660631674No Hit
CAGTAATACAGAGCAGTGTCCTCACTTCGCAGGCTGCTCATCTGAAGAAA22710.1208168945748905No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGA22370.11900809914752535No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGCGGCCCTGGT21590.11485850963768765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21540.11459251031013394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21260.11310291407583323No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCTTCGGAGGCCAGTTAGTAGTCTC20040.10661253048352293No Hit
GTATTACTGTGTGAAAGACCTCGGGTACGGCTCCCTCCTTCACTATTGGGGCCAGGGAACCCTGGTCACCGTCTC19660.10459093559411481No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19060.10139894366347042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36600.039.2461
GGTATCA16000.036.1684341
TATCAAC41050.034.987842
ATCAACG41950.034.15513
TCAACGC42050.033.991974
CAACGCA42850.033.3573465
AACGCAG44650.032.091446
AGAGTAC45350.031.76658811
CGCAGAG47150.030.4052478
GCAGAGT49400.028.9529449
CAGAGTA50250.028.60036310
AGTACGG44250.028.42831813
GAGTACG45350.027.73876812
ACGCAGA54800.026.345877
TACGGGT3750.024.81507715
GTACGGG50550.024.74896214
GTGGTAT11200.024.6043781
CTATACG700.001347200124.601764
TGTTAGA7650.023.23092870
TACGGGG26100.022.97692315
Produced by FastQC (version 0.11.5)