FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634648
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39500.2416422373501818No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33230.2032853556239631No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG29680.18156814188742776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29120.17814232788955175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28920.17691882289031033No Hit
GTAGTATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA28360.17349300889243432No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26920.16468377289789607No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26220.16040150540055106No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24890.15226519715559558No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24290.1485946821578713No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24240.14828880590806096No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23090.14125365216242275No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAT22920.14021367291306752No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAA21680.13262794191777066No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21220.1298138804195154No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21040.1287127259201981No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT20610.12608219017182903No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19990.1222893246741806No Hit
CAGTAGTATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA19930.12192227317440818No Hit
ATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19280.11794588192687355No Hit
ACATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCA19200.11745647992717698No Hit
GGCCAAAAGTGTACGGAGGGAGATTATCATAGTGTTGACAGTAGTATGTTGCAATATCTTCAGGCTGCAGGCTG19010.11629415017789763No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18860.11537652142846655No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18640.114030665929301No Hit
ATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18420.11268481043013542No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17940.109748398431956No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17720.10840254293279042No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17360.10620023393415585No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16800.10277441993627985No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16750.1024685436864695No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16640.10179561593688673No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16360.10008270893794873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27650.038.0370981
TCAACGC32250.032.268164
ATCAACG32800.031.8321343
CAACGCA32800.031.7270775
TATCAAC33050.031.4870832
GTGGTAT5000.031.034531
AACGCAG34450.030.2074976
GTATTAA756.186035E-527.586251
AGAGTAC38300.027.43836411
ACGCAGA38600.027.1358497
CAGAGTA38850.027.0499210
CGCAGAG39850.026.3711248
GCAGAGT41300.025.3618349
TGTTAGA10750.022.98774170
AGTACGG36900.021.94313213
GAGTACG37550.021.56329212
GCGTAGA24100.020.50773470
CAGTTCG20250.019.71313370
GAGTACT12400.019.45062312
GTGTATA3450.018.9754313
Produced by FastQC (version 0.11.5)