FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634648
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35970.22004737411357062No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31320.19160088288120747No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG30630.18737979063382454No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26300.16089090740024764No Hit
GTAGTATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA26010.15911682515134756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25210.15422280515438186No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24560.15024641390684723No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23890.14614767215938845No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAT23240.14217128091185383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22370.1368490341651536No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21710.1328114676676569No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21550.13183266366826374No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAA21500.1315267874184534No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21270.13011975666932574No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20190.12351282967342203No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20160.12332930392353583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19850.12143287117471163No Hit
ACATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCA19460.11904703642619083No Hit
CAGTAGTATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA19180.11733412942725285No Hit
ATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18670.1142141916791872No Hit
GGCCAAAAGTGTACGGAGGGAGATTATCATAGTGTTGACAGTAGTATGTTGCAATATCTTCAGGCTGCAGGCTG18650.11409184117926308No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT18020.11023780043165257No Hit
ATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17870.1093201716822215No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17470.10687316168373863No Hit
AGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAACCAGGGAA17180.10509907943483858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16690.10210149218669708No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16490.10087798718745565No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16350.10002153368798665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33250.038.5020521
TATCAAC37700.033.7675822
TCAACGC39250.032.3464244
CAACGCA39600.032.1474235
ATCAACG39950.031.7796553
AACGCAG40400.031.5998696
GTGGTAT5750.031.1220441
AATAGCG2500.029.4536862
ACGCAGA43950.029.058997
AGAGTAC44250.028.7151811
CGCAGAG44950.028.4203458
CAGAGTA45350.028.17053410
GCAGAGT48150.026.6754029
TGTTAGA10100.026.57788570
ATACCGG1000.00998932826.1889470
AGTACGG40800.025.0665613
TACGTCG700.001355366124.5760215
TTAACGG2250.024.54864335
GAGTACG41600.024.50173612
TAACGGC2350.023.5097936
Produced by FastQC (version 0.11.5)