FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255630
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38990.3105214115623233No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33820.2693468617347467No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33110.26369232974682033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31560.2513479289281078No Hit
GTCTCTGGTGACTCCATCAGCAGTGGTGATTACTACTGGACTTGGATCCG28690.22849087708958848No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25650.20427992322579105No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25320.20165176047083933No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25150.2002978584455612No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC25050.1995014454895152No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24620.19607686977851754No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC24350.19392655479719345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24040.19145767463345095No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT23580.18779417503563947No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23480.1869977620795935No Hit
GGCTAACAGTTTCCCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22870.1821396430477131No Hit
GTAGTAGGTGGTCCCACTGTAATGGATGTTCCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAAGT21560.1717066333235109No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21530.17146770943669712No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21490.1711491442542787No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21380.17027309000262816No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21110.16812277502130404No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT20500.16326465598942363No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGAC20210.16095505841689034No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20150.16047721064326276No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20010.1593622325047984No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19210.15299092885643065No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18980.1511591790575249No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTGGTGATTACTACTGGA18960.1509998964663157No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18910.15060168998829274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18800.14972563573664216No Hit
GAGTAATCTCGGACCACCACATCTCTGGCACAAAAATACACGGCCGTGTC18640.14845137500696862No Hit
ACTACACCCCGTCCCTCAAGAGTCGACTTACAATATCCGTAGAAACGTCCAAGGACCAGTTCTCCCTGAATCTGA18520.14749567945971345No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTGTTGCCAGCTGGTTAGTCTGGTATCAGCAGAAA18270.1455046470695985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17880.14239863654101925No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGACTTACAATATCCGTAGAAACGTCCAAGGACCAGTTCTCCCTGAAT17840.14208007135860087No Hit
GATCTATGGTGCAGCCACTTTGCAACTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA17480.13921298471683538No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17200.13698302843990667No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17130.1364255393706745No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17040.1357087677102331No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16860.13427522438935038No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16700.1330009636596768No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16520.13156742033879407No Hit
GGGTTCCCTGGCCCCAGTAGTCGTCTACCCAATAATGTCCCGAACCAGAGTAATCTCGGACCACCACATCTCTGG15960.12710750778493665No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15900.12662966001130907No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15790.1257536057596585No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15730.1252757579860309No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15670.12479791021240334No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15410.12272723652668382No Hit
GGGTGTAGTAGGTGGTCCCACTGTAATGGATGTTCCCAATCCACTCCAGG15390.12256795393547464No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15370.12240867134426542No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15340.12216974745745163No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14940.11898409563326777No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14920.11882481304205857No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14910.11874517174645398No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14780.11770983490359421No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14780.11770983490359421No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14760.11755055231238501No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14720.11723198712996663No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCT14610.11635593287831607No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14610.11635593287831607No Hit
GTGTAGTAGGTGGTCCCACTGTAATGGATGTTCCCAATCCACTCCAGGCC14520.11563916121787468No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14490.1154002373310609No Hit
GTAATGGATGTTCCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAAGTCCAGTAGTAATCACCA14380.11452418307941034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14310.11396669401017814No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13900.11070140089038968No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13880.1105421182991805No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC13820.11006427052555291No Hit
ACCCAATAATGTCCCGAACCAGAGTAATCTCGGACCACCACATCTCTGGC13820.11006427052555291No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13630.10855108590906556No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTC13500.10751574906620581No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCTCGAACTTT13490.10743610777060122No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13410.10679897740576444No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13370.10648041222334605No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13210.10520615149367249No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13130.10456902112883572No Hit
ACCTTGATGGGACCCCAAGTTGCAAAGTGGCTGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13120.10448937983323113No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTCGAGGGAAA13070.10409117335520814No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCC12990.10345404299037138No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12780.10178157578267483No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12780.10178157578267483No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12730.10138336930465185No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG12560.1000294672793737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2600.050.3382871
GTATCAA16100.041.715451
TATCAAC19200.034.61312
ATCAACG20250.032.9883883
TCAACGC20750.032.3594324
CAACGCA21000.031.9742015
GGATTAT850.00377662731.95773970
AACGCAG21500.031.3957756
CCCTAAA450.00461402930.67863835
TCAGTAT450.004636596430.64805830
CTTGTAT450.004654712530.62363416
ATAACCC703.90213E-529.5146483
CGCAGAG22900.029.4904778
AGAGTAC22700.029.14431611
GCAGAGT23800.028.3764219
ACGGGTA500.007788365227.56127216
TTCGGGG11850.027.50792770
TGATTCG550.00819033727.27826368
TGTTAGA7900.024.92905870
ACGCAGA27550.024.8851787
Produced by FastQC (version 0.11.5)