FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529219
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGATGTGTCTACGGA89960.5882741451682199No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTACTTACTTCTGGGGCTGGATCCG64120.4192990016472461No Hit
GAGTAACCCCTTCCGCCGGTCCCAGTCAAGCGGAGTCTCGCACAGTAATA52640.3442280013523243No Hit
GTGTATTACTGTGCGAGACTCCGCTTGACTGGGACCGGCGGAAGGGGTTACTCTGACCACTGGGGCCAGGGAACC52570.34377025135052597No Hit
GTTTTATACAGGTCCGATAATAAGAACTACTTAGGTTGGTACCAGCAGAA48370.31630525124262776No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44080.2882517154181317No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT43980.2875977868441342No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACTTACTTCTGGG43500.2844589296889458No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42640.27883514395256664No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT41870.2737998939327853No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38630.2526126081352638No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC38540.252024072418666No Hit
GGGTTACTCTGACCACTGGGGCCAGGGAACCCTGATCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT38070.24895060812087738No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG37090.24254210809570115No Hit
CAGTAATACACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGCTTCAGGGA35450.23181767948214088No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35430.2316868937673414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34650.22658625089016027No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34560.22599771517356243No Hit
ATATTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT32690.21376925083980777No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG31230.20422189365944315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31100.2033717865132463No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29190.19088175074989258No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA28010.18316539357672118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27370.1789802507031367No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27200.1778685721273408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26910.17597217926274786No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26740.17486050068695197No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACCTAAGTAGTTCTTATTATCGGACCTGTATAAAACACT26540.17355264353895683No Hit
ATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA25290.1653785363639871No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC25050.16380910778639293No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24440.1598201434850077No Hit
GGGTTCCCTGGCCCCAGTGGTCAGAGTAACCCCTTCCGCCGGTCCCAGTCAAGCGGAGTCTCGCACAGTAATACA23890.15622353632802102No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC23840.15589657204102225No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA23760.15537342918182417No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACATCC23720.15511185775222516No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23660.1547195006078266No Hit
CTCCTAAGCTACTCATTTACGGGGCATCTACCCGGGAATCCGGGGTCCCT23650.15465410775042684No Hit
GTCCGTAGACACATCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCTGACACGGCTGTGTATTA23630.15452332203562735No Hit
GTCTACGGACATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGCTCTCATCATAATAGAGACTCCCAAT23560.15406557203382903No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22960.15014200058984356No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT22960.15014200058984356No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22930.1499458220176443No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22510.14719932200685448No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA22420.14661078629025664No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22190.14510675057006223No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21810.14262182198887144No Hit
CTATTATGATGAGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACATCCAAGAA21750.1422294648444729No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACTTACT21500.14059464340947897No Hit
GTATTACTGTGCGAGACTCCGCTTGACTGGGACCGGCGGAAGGGGTTACTCTGACCACTGGGGCCAGGGAACC21420.1400715005502809No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20870.13647489339329424No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20840.13627871482109494No Hit
CTATAATATTGTTGACAGTAATAAAGTGCCACATCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTC20670.13516703624529908No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20620.13484007195830028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20060.13117807194391384No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19960.13052414336991627No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19680.12869314336272306No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19630.12836617907572428No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19540.12777764335912645No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19400.12686214335552987No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19380.12673135764073035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19100.12490035763353712No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA19030.12444260763173882No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG18760.12267700048194535No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT18720.12241542905234633No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18370.12012667904335482No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC18300.1196689290415565No Hit
GGACTGGTGAATCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACTTA18250.1193419647545577No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGATTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAG18240.11927657189715797No Hit
GTCATGGATCTCATGTGCAAGAAAATGAGGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCGGATGGGTC18190.11894960761015917No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17830.1165954647437679No Hit
ACTTAGGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCATT17820.11653007188636814No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17730.11594153616977032No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCCGTAAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA17700.11574535759757104No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17680.11561457188277154No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17560.11482985759397443No Hit
GGACTGGAGTGGATTGGGAGTCTCTATTATGATGAGAGCACCTACTACAA17500.1144375004495759No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC17370.11358739330337905No Hit
GTAATAAAGTGCCACATCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17280.11299885758678122No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAGGCACCTGTGGTT17280.11299885758678122No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG17240.11273728615718219No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG17080.11169100043878608No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAATCCTTCGGAGACCCTGTCCCTCACCTGCA16930.11071010757778971No Hit
ATTATGATGAGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC16450.10757125042260134No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC16430.10744046470780183No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG16400.10724428613560257No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16260.10632878613200596No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG16250.1062633932746062No Hit
AACCAGGACAGCCTCCTAAGCTACTCATTTACGGGGCATCTACCCGGGAA16210.10600182184500717No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC16190.10587103613020764No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT16110.10534789327100959No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG16090.10521710755621005No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA15960.10436700041001322No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15950.10430160755261345No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15900.10397464326561466No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15840.10358228612121613No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15820.10345150040641661No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAATCCTTCGGAGACCCTGTCCCTCA15650.10233982183062074No Hit
GTCCTGGTTTCTGCTGGTACCAACCTAAGTAGTTCTTATTATCGGACCTG15430.10090117896782606No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC15390.10063960753822702No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15360.10044342896602776No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG15330.1002472503938285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2150.052.9339031
TGGTATC2250.052.0782662
GTCACGA200.00738839951.6987212
GGTATCA8100.045.131451
GTATCAA20450.041.8231541
CTTATAC503.8534217E-641.3846861
ACTAGGG350.001354978539.3869253
TATCAAC23200.036.6918072
TCAACGC24750.034.672434
CGTCAGA1300.034.463565
ATCAACG24850.034.394223
CAACGCA25250.033.9858445
AACGCAG25600.033.655826
CTGGAGT32550.032.13432370
CTACGGA24050.032.0029669
ACCTAAC450.004647125530.6342743
CGCAGAG28200.030.55388
AGAGTAC29100.029.72824311
GCAGAGT29550.029.1588949
GACAGGT2800.028.3288021
Produced by FastQC (version 0.11.5)