FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529219
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGATGTGTCTACGGA84030.5494961807301636No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTACTTACTTCTGGGGCTGGATCCG59830.39124546582275005No Hit
GTGTATTACTGTGCGAGACTCCGCTTGACTGGGACCGGCGGAAGGGGTTACTCTGACCACTGGGGCCAGGGAACC50240.32853371557638245No Hit
GAGTAACCCCTTCCGCCGGTCCCAGTCAAGCGGAGTCTCGCACAGTAATA48700.3184632155368198No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT43710.2858321796943407No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT43040.281450858248557No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42620.2787043582377671No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACTTACTTCTGGG40700.26614892961701364No Hit
GGGTTACTCTGACCACTGGGGCCAGGGAACCCTGATCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT38120.24927757240787618No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT38060.24888521526347765No Hit
GTTTTATACAGGTCCGATAATAAGAACTACTTAGGTTGGTACCAGCAGAA37740.2467926438266854No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC34650.22658625089016027No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG34270.22410132230896948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34130.2231858223053729No Hit
ATATTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT32840.21475014370080414No Hit
CAGTAATACACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGCTTCAGGGA32470.2123306079770131No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32190.2104996079698199No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29830.19506689362347707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28700.18767750073730446No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27600.18048428642333114No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27100.17721464355334324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26930.17610296497754735No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26260.1717216435317636No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26250.17165625067436385No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA24900.16282821492539656No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24420.1596893577702082No Hit
GTCCGTAGACACATCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCTGACACGGCTGTGTATTA24290.15883925062401136No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23870.15609275061322153No Hit
GGGTTCCCTGGCCCCAGTGGTCAGAGTAACCCCTTCCGCCGGTCCCAGTCAAGCGGAGTCTCGCACAGTAATACA23860.15602735775582177No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACCTAAGTAGTTCTTATTATCGGACCTGTATAAAACACT23780.15550421489662372No Hit
CTATAATATTGTTGACAGTAATAAAGTGCCACATCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTC23600.15432714346342805No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23570.1541309648912288No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23520.15380400060423No Hit
GTAATAAAGTGCCACATCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA23230.15190760773963705No Hit
GTCTACGGACATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGCTCTCATCATAATAGAGACTCCCAAT23230.15190760773963705No Hit
ATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA22960.15014200058984356No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22860.14948807201584602No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT22110.14458360771086418No Hit
CTCCTAAGCTACTCATTTACGGGGCATCTACCCGGGAATCCGGGGTCCCT21990.1437988934220671No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT21950.14353732199246805No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21800.1425564291314717No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA21690.14183710770007435No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21550.14092160769647774No Hit
TCTTCAGCCTGTAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21400.13994071483548137No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21340.1395483576910828No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACTTACT21080.13784814339868914No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21040.1375865719690901No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20950.1369980362524923No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACATCC20950.1369980362524923No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20940.13693264339509253No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20840.13627871482109494No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT20790.13595175053409617No Hit
CTATTATGATGAGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACATCCAAGAA20770.13582096481929665No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20220.13222435766230997No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20130.13163582194571216No Hit
GTATTACTGTGCGAGACTCCGCTTGACTGGGACCGGCGGAAGGGGTTACTCTGACCACTGGGGCCAGGGAACC20020.13091650051431483No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19990.13072032194211555No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19890.13006639336811798No Hit
GTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAATCC19820.12960864336631966No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19470.12731989335732816No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA19150.1252273219205359No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19130.12509653620573638No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA19020.12437721477433906No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA18970.12405025048734028No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18500.12097678618955165No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT18410.12038825047295384No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAGGCACCTGTGGTT18180.11888421475275941No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA18110.11842646475096111No Hit
GGACTGGAGTGGATTGGGAGTCTCTATTATGATGAGAGCACCTACTACAA18000.11770714331956378No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG17990.11764175046216402No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17960.11744557188996475No Hit
GGACTGGTGAATCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTACTTA17840.11666085760116765No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA17840.11666085760116765No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17830.1165954647437679No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT17810.11646467902896838No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17810.11646467902896838No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17680.11561457188277154No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17490.11437210759217614No Hit
GTCATGGATCTCATGTGCAAGAAAATGAGGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCGGATGGGTC17450.11411053616257712No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17370.11358739330337905No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC16840.11012157186119188No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16790.1097946075741931No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAATCCTTCGGAGACCCTGTCCCTCACCTGCA16590.10848675042619794No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC16570.10835596471139844No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16550.1082251789965989No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC16430.10744046470780183No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAATCCTTCGGAGACCCTGTCCCTCA16320.10672114327640449No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16260.10632878613200596No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16210.10600182184500717No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGG16170.10574025041540813No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCCGTAAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA16060.10502092898401079No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16000.10462857183961224No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG15920.10410542898041418No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGATTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAG15750.1029937504046183No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG15480.10122814325482483No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15460.10109735754002533No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC15340.10031264325122825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2650.054.5607031
ACCGTAT200.00731219751.83445438
TGGTATC3000.049.33152
GGTATCA8400.042.6216851
GTATCAA22650.041.9485931
TATAAGA1550.037.7480352
TATCAAC26000.036.402942
ATCGTTG601.3273246E-534.5585740
TGTCGAT400.002597556434.4974131
GTATAGG501.8855992E-434.425211
ATCAACG27750.033.8592073
TCAACGC28100.033.3149954
GACAGGT2200.032.8604241
CAACGCA28700.032.7384345
AACGCAG28850.032.5735446
GTATAAG1800.032.5126951
ATAAGAG1700.032.3927763
GGCTTAG751.6982431E-632.130191
TAAGAGA2000.030.9755944
GCCATAA450.00467250530.6001831
Produced by FastQC (version 0.11.5)