FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122009
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCGTGCTTGTATATGAA38980.3474125430366423No Hit
TCTCAAGACTCCGGGCTGAGGACGAGGCTGATTATTCCTGCAGTTCATAT36070.3214769221993763No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGC35900.31996178283774906No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG35460.3160402456664786No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35360.31514898721846263No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35350.315059861373661No Hit
GTCTTGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG33340.2971455665685391No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30740.27397284692012275No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT29530.26318861969912893No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28960.2581084465454377No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28910.25766281732142965No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC27300.24331355630837184No Hit
GTTCATATACAAGCACGCGCACGCTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG25270.2252210098136468No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAATGC24550.21880394898793148No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24280.21639755117828824No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24010.213991153368645No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23550.20989136450777135No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA23260.2073067150085249No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23100.20588070149169926No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22520.20071140249320638No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22110.19705724285634074No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20640.18395574367050532No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20580.1834209886016957No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20340.18128196832645727No Hit
GTATATGAACTGCAGGAATAATCAGCCTCGTCCTCAGCCCGGAGTCTTGA19750.1760235434831628No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18710.16675445562379623No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT18680.16648707808939145No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18130.16158515662530334No Hit
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA17720.1579309969884377No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17520.15614848009240567No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17430.15534634748919127No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17330.15445508904117525No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17120.15258344630034162No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16650.14839453159466637No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCA16480.1468793922330391No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCG16200.14438386857859428No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16010.14269047752736386No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15970.14233397414815746No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15750.14037320556252222No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT15490.1380559335976806No Hit
CTTGTATATGAACTGCAGGAATAATCAGCCTCGTCCTCAGCCCGGAGTCTTGAGATGGTCAGGGAGGCCGTGTTG15270.13609516501204535No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15130.13484740318482294No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGAAA15110.13466915149521974No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14610.13021285925513967No Hit
CTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC14520.12941072665192524No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14300.12744995806629003No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13940.12424142765343237No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13730.12236978491259873No Hit
ATATACAAGCACGCGCACGCTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGC13600.12121114893017793No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13410.11951775787894749No Hit
CCACCACACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAT13290.11844824774132827No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC13110.11684398253489946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13090.11666573084529626No Hit
GTGTATTACTGTGCGAAATCGGAAATTGCCCTCCGTACTGGGAGCTACTA13040.11622010162128825No Hit
GATTATTCCTGCAGTTCATATACAAGCACGCGCACGCTCGTGGCTTTTGG12490.11131818015720017No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG12470.11113992846759696No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12470.11113992846759696No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGC12300.10962478910596975No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12160.10837702727874732No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12140.10819877558914412No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT12120.10802052389954091No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12060.10748576883073131No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTC12040.1073075171411281No Hit
CTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGG11850.10561412608989768No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA11840.10552500024509606No Hit
CACCCAGGCAATGCCCCCAAACTCATAATTTATGAGGTCAGTAATCGGCCCTCAGGAGTTTCTGATCGCTTCT11730.10454461595227846No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC11620.10356423165946084No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11580.10320772828025443No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA11560.10302947659065124No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11530.10276209905624642No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11470.10222734398743683No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11380.1014252113842224No Hit
CTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC11230.10008832371219839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT200.00746138651.5694357
TACCGAA200.00746138651.5694357
GTGGTAT3800.047.026931
TGGTATC3850.045.5215452
GTATCAA27550.044.5321351
GGTATCA10050.039.666041
TATCAAC31550.038.884492
TCAACGC32900.037.393384
ATCAACG33000.037.2800673
AACGCAG33650.037.0738646
CAACGCA33450.036.881275
CGCAGAG36150.034.3351368
CTAACGA5400.034.16520369
AGAGTAC36650.033.9605311
ACGCAGA36750.033.7715537
CAGAGTA37700.033.01468310
GCAGAGT38050.032.7119
CGATTAA850.00394662831.67106470
CTATTAG553.3322457E-431.241671
GTATGAT450.00467166930.60059530
Produced by FastQC (version 0.11.5)