FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731850
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16580.226549156247865No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16150.22067363530778164No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15680.21425155428024867No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC15470.21138211382113822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11560.15795586527293845No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11370.1553597048575528No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11270.15399330463892874No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10860.1483910637425702No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10540.1440185830429733No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTG10480.14319874291179885No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10280.1404659424745508No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10230.13978274236523877No Hit
GCTTAATAGTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10080.13773314203730272No Hit
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA9900.13527362164377946No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9470.12939810070369612No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9320.12734850037576007No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9250.12639202022272322No Hit
GTATATGGTACTACCACTACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT8920.12188289950126392No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8910.12174625947940151No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8890.12147297943567671No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC8720.11915009906401586No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA8580.11723713875794221No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8230.11245473799275807No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT8110.11081505773040924No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT7860.10739905718384914No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7830.10698913711826195No Hit
GGCCAAAAGTGTACGGATAACTATTAAGCTGTTGACAGTAATAAGTTGCA7800.10657921705267473No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7730.10562273689963791No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7700.10521281683405069No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7660.10466625674660109No Hit
GTTTATTACTGTGCGAGAGATTCACGATGGGGTATGGACGTCTGGGGCCA7500.10248001639680263No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7420.10138689622190339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA252.5262407E-455.37722840
CGCTTAA350.001323747639.57143841
CACGCTT350.00132866439.54161539
GTGGTAT2300.039.010241
GTATCAA10450.037.3160131
TATCAAC12200.031.669622
TCAACGC12800.030.185114
ATCAACG12750.030.0329173
CGAACTA703.8332844E-529.589329
AACGCAG13300.029.0523176
CAACGCA13200.029.0090665
AGAGTAC13550.027.77333811
GCTTAAC500.00757494427.71711242
CAGAGTA14050.027.03069710
GTCATAG5750.027.0070861
CGCAGAG14400.026.8513878
ATAGGTG5300.026.0356734
TCATAGG5600.025.872952
CATAGGT5500.025.7161463
TGTGTCG5200.025.0556570
Produced by FastQC (version 0.11.5)