FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length52-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATCAGTGGCCCTGATCTGCCTCCTGCGCTACGGGCAGCTCCTGGAGCAGAGTCGGCACTCTTGGGTTAACA11.3888888888888888No Hit
GTATATGCAAGTCCACCTGAAATATGAGTTTGACATCGAGTATGACATTC11.3888888888888888No Hit
GGTTAGGAGGTGGGGCAGTTTAGAGGGAAGAAGAGTGGGACACCCCCAGGGGAGTCAAAGCAGGCCTGGCATCGA11.3888888888888888No Hit
AACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTCTCAAGT11.3888888888888888No Hit
ACTTGATCTTAGCCAAAAGGCCGAGAAGCGATCCCGTACTCTGCGTTGATAC11.3888888888888888No Hit
GGAGTAGCTGGTCAGAGACGAGCAGCCCTGAAAACTGTTCCTGCCCTGGC11.3888888888888888No Hit
TCACCCTCCTTAACCTCTACTTCTACCTACGCCAAATATACTCCACCTCAATCACACTACTCCCCATATCAAACA11.3888888888888888No Hit
ATACAAATGATAACAAGAAGGAATGCAGCAAAATAATCCAAAGCAAAAGCATCAGCAGCAGCACAACAACACAAA11.3888888888888888No Hit
ACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGATAGATGAGCTGGAGTACAGCAATAGGGGT11.3888888888888888No Hit
TGCTGTCAGAGGGGATGGGGGTGACATCCTCAATCCGCCCGATCTTCATACCCGAGCGGGCAAGGGCTCTGAG11.3888888888888888No Hit
GCCCAGGGACGGGGCTGGGTGGCCTTGGTGGGTCCCTGTGAGGTGCCTGC11.3888888888888888No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGTCTCCCACTCAGGAAACACAGCTTCCTTGACGATCATTGGGGAT11.3888888888888888No Hit
CAGCCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTT11.3888888888888888No Hit
CACAAAGAGGGCGGGAGAACTCACTGAGGATGAGGTGGAACGTGTGATCACCATTATGCAGAATCAACGCCAGTA11.3888888888888888No Hit
AGTGACTTTTGTTGGGGTCCCTAGATAGGCTCAAGATGGGGCTCATCACA11.3888888888888888No Hit
GTATCAACGCAGAGTACGGGAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT11.3888888888888888No Hit
GTTCCACCCGGCCATGGCGCTGCTACTGGGGCTGCTCTCGGCGCTGCTGCTGGGGCCGCGGACTGGGCTCGAG11.3888888888888888No Hit
ATGCGTTGGCGGCGCAACCCGTTGATGCCCGTTTGATCATGAAGCAAGGCGGCTCGGCGCATATCGCGATCATCT11.3888888888888888No Hit
GCTGAGGGATGCCCAAGAAGTTCCAGGGTGAGAACACCAAGTCGGCAGCG11.3888888888888888No Hit
ACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGG11.3888888888888888No Hit
GGTCTACACTGCTTTTGTGAGCTGTAACAGTCACGGTGAAGAGCTGCAAC11.3888888888888888No Hit
CTACGCACACGAGAACATGCCTCTCGCAAAGGATCTCCTTCATCCCTCTC11.3888888888888888No Hit
CCAGGGCGCCGGCCCAGCATGGACAGTTGCTCATTGTTGGAGATAAGGTCGCGCTGGTCATCCATGACTGGCTT11.3888888888888888No Hit
CAAAAATAAAAAGAAAAAATCTAAAAACTATTAAACCTTATACTGAATTAAAAAAAATTTTTTAAGACAGAGTGT11.3888888888888888No Hit
GCATTCCTCCAAGTGTTCCAAATACCAGACCTCATTCACGTTGGTACCAG11.3888888888888888No Hit
GAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAAC11.3888888888888888No Hit
CCCCTGAAGGTGTACCCGCCACTGAAGGGGAGCTTCCCGGAGAACCTGAG11.3888888888888888No Hit
CTTTTGGCCCTTGTACTTCTTGGCCAGGCTGTTGTACACGGGCTCTAGCT11.3888888888888888No Hit
GGTTGGGCAGCCAGTCTGGGCCCTTATCAGTATCCTCTTCTCACTCCTCATCCTCTTCCTTGACAGCCTTTACTT11.3888888888888888No Hit
CTCGGCGTGCAAGCCGCCCCGCAGGTGCTACCGTCTCTCACCTCCCTCTA11.3888888888888888No Hit
GTATAGCTGGAACACATCCCGGAGACTGGCCCTCTTGTGCCCTTGCTTGGTCACGTAGGATAGACATGCCTCTTG11.3888888888888888No Hit
GGCCTGCTTCTTGGGCTGTTTCAGTGGCTTCTTCTTGCCACCTTCGCGGCCGGACATGGCGCCTGCCGCCGCTTC11.3888888888888888No Hit
CCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACTGAGCAGG11.3888888888888888No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAATTGTCAGGTAGG11.3888888888888888No Hit
GCAGATAGTGAGGAAAGTTGAGCCAATAATGAAGTGAAGTACGTGGAAGC11.3888888888888888No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGACCCTCTAAGAAGTTGGGGGACGCCGAC11.3888888888888888No Hit
GACCCAGACCCTGCAGCACCACAACCTCCTGGTCTGCTCTGTGAATGGTT11.3888888888888888No Hit
GGCTCGCAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCTACCACCCTGACTGCATCAGTAGGAGACACA11.3888888888888888No Hit
TCCATGTCCTGGTAGGACTTCTTCATGCTTTGGGAAGCAAGCTCAATGGT11.3888888888888888No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA11.3888888888888888No Hit
CCCTGCGTCCCGCCGTCATCTTCCTCTCTCCCTGCCAGGTCTCCGGGCTGCTGAAGAGGCGCTTTCACTGGACCA11.3888888888888888No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGA11.3888888888888888No Hit
ATGCGGCGCACCTTGGCTTGCCGGGCCTTACGTCTGCGGATCTTACGGGCCGGCTGGTTGAACCACGTGGCCACG11.3888888888888888No Hit
TGGTGGTTCCGCGATGGTAGGCGGCGGCGGGGTCGGCGGCGGCCTCCTGGAGAATGCCAACCCCCTCATCTACCA11.3888888888888888No Hit
CACCAAGAGGGCGGGAGAACTCACTGAGGATGAGGTGGAACGTGTGATCACCATTATGCAGAATCCACGCCAGTA11.3888888888888888No Hit
GTGTTGGGCTGACCCAGTGTCACGGGAGACTCGGAAAACACTGTGACCTC11.3888888888888888No Hit
GCCTTAAGGACACCCGCACTGGCGACACGCTCTGCGATCCGCAGAAGGCAGTGATCCTGGAACGGATGGAGTTCC11.3888888888888888No Hit
CCTCTAGAGCCTTCAGCCTTAATGGAAGGTTGGATCCAAGGAGGGTGTAATGGAGCATCAAGCCACTCGGCGGCA11.3888888888888888No Hit
ACAATAAACTCAACATTCGGCCACAACACCCAGCACAGCATCATCTACTGCGACAAGTGATCCAAATGAGAGGTG11.3888888888888888No Hit
CCCCTTCCTCCTTCCAGAAAATGAGCTTCCCCTCCTTGCTGTTCCCTGCT11.3888888888888888No Hit
CAGGTATCAACGCAGAGAAATTAATATTTAATTTATAATTTTTATTTTTTTTGGAAAT11.3888888888888888No Hit
GAAATAGCCTCCGCGTAAGACCGTACCCGAAACCGACACAGGTGGACTGGTAGAGTATACCAA11.3888888888888888No Hit
GTATTGTTATACCCAAGGTCTTCTTGGTTCTAGAAAGCCGAACCATGCTAGTAGCCAGTGGAATTTAAGGAGACC11.3888888888888888No Hit
CTGTTGAACTCTCTAAAGGCCATTTGTTCCAGGGCCTAGCTGGGCTTGTG11.3888888888888888No Hit
TTGATTCATACTAACAATCACAATTAATATATCAAATTAACTCTGACAATATAAAATGAGTTAACACATTCTCTT11.3888888888888888No Hit
CAATTACAAATGCTGTAGCAAAACGGTTATCCATTCTGGCATTCAGACTGCTCGCCCATCCTC11.3888888888888888No Hit
TCCTTGGGGAGTGATGCTGGGGAAAGGGAAGAGAGTGGCTCAGCCTGCAGGTAAATAGGCTAGAAAAGCCAAGGC11.3888888888888888No Hit
CAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG11.3888888888888888No Hit
GCACGTGAAACTCTCCCCGGAATTCAAGTCATCCTCGCAGATGCTGGCCTCACACACGGCGCTGAAAGTGCCATA11.3888888888888888No Hit
GTGTTGGATAGAAGTTATTCAGCAGGCTCACAACAGAGGAAGTTCCATAT11.3888888888888888No Hit
CAGAAAGAGTCCACCCTGCACCTGGTGTTGCGCCTGCGAGGTGGCATTATTGAGCCTTCTCTCCGCCAGCTTGCC11.3888888888888888No Hit
GTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGT11.3888888888888888No Hit
GAGTGGTTTTTCCTTCTTGATGGCTCAATGACAGAGACTAGCTCGTAAACTCCGGGCCGTTTCCGGGCTGTTCG11.3888888888888888No Hit
TTTTCGATGGTGTCACTGGGCTCCACCTCAAGGGTGATGGTCTTGCCGGTAAGGG11.3888888888888888No Hit
CTTCACATCCCCTCTCTGATGAACACACCCGCCCTGCTTGGCTATTTGATCACTCACAGTGGCCTAAGAGGAATA11.3888888888888888No Hit
CAAGAAGATGAAAGGATTGTTCTTGTTGACAACAAATGTAAGTGTGCCCG11.3888888888888888No Hit
GTGGAAGCAGGATAAAGACCTCCAAACGGCAAACTACAATGATATTGACAAAAAGCACCACAGCAAGGACAGAA11.3888888888888888No Hit
GCGCCATCCTGCGCAGCCAGAAGCCTGTGATGGTGAAGAGGAAGCGGACCCGCCCCACCAAGAGCTCCTGAGCCC11.3888888888888888No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACACAGAGCTTCAACAGGGGAGAGTGTTAG11.3888888888888888No Hit
GGATTACAGGCGTGAGTCCCTGTGCCCAGCCTCCAGCCTTGTTCTTTTTGCTAAGGATTGCTTTGGCTGTTCTGG11.3888888888888888No Hit
CCTCATGGGCCACCACGCAGGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGG11.3888888888888888No Hit
GGGCAACACAGTGAGACACTGTCTTTGCAAAACAAACAAACCAAAATTAGCCAGGCATGGTGGTGAGTGCCTGTA11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACA50.057.034
AGCCTCC50.057.06
GTAGAGT50.057.050
AAACCGA50.057.030
GCCTCCG50.057.07
AACCGAC50.057.031
TATACCA50.057.056
GAAACCG50.057.029
GAAATAG50.057.01
TGGTAGA50.057.048
GGACTGG50.057.044
GTACCCG50.057.023
TAGCCTC50.057.05
AAATAGC50.057.02
ACCGTAC50.057.020
TAAGACC50.057.016
ACCCGAA50.057.025
AGGTGGA50.057.040
ACACAGG50.057.036
ACCGACA50.057.032
Produced by FastQC (version 0.11.5)