FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length52-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAGTTTTACGGTC11.3888888888888888No Hit
GCATGCCCGCCTCGCCCTGAGTGATGCCATCGCACATCGCTGGCACCCCTGCCGCAAACTGAGCGGTCGCACCTG11.3888888888888888No Hit
GGGCTCAGGAGCTCTTGGTGGGGCGGGTCCGCTTCCTCTTCACCATCACAGGCTTCTGGCTGCGCAGGATGGCGC11.3888888888888888No Hit
ACGTACAGCAACCCTGTGTCGGTGCCCCCTGCCCGCTTCTCTAGTGTCCT11.3888888888888888No Hit
GGACAAGGCATAGCAGAAAGTACGGTGGTACACTCAAGGCACTCTGAACA11.3888888888888888No Hit
AAAGACCGTATCAATAGCAAATTCTCCACAGCTAAAGAATACGCCTCAAC11.3888888888888888No Hit
GCTTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTT11.3888888888888888No Hit
GGACGCAGGTTGTTGGTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCGAGCATAGCA11.3888888888888888No Hit
CTACTACGTTCAACTCCTCTAGCGTCAGTGGATGCTCCGGGTCATTGATG11.3888888888888888No Hit
GGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGTGCTGACCCAGGACTGTGAC11.3888888888888888No Hit
GCCATGGACCCAGCCCCAGAGCCATGAGATGAGCTAGTCCAAATTTGGGCTCATTACTGGCCCACAAAGGTTTGA11.3888888888888888No Hit
CCGCCAGCCGAGGCAGCTCGAGCCCAGTCCGCGGCCCCAGCAGCAGCGCC11.3888888888888888No Hit
CACTGGAGTATCAGCGTGAGGCCAATGATAATGCAACAACCAAAGCAAAAATCCTTAGTACCATGGAGCTTGCTG11.3888888888888888No Hit
GCTGTCGGGAAGATCAGAAGCCAGTCATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGTCCATG11.3888888888888888No Hit
AAAGAGGGCCACACCCCCCTCCAGTGTTCAGCAACCTGGAAGCTCAGCAG11.3888888888888888No Hit
CACAAGAGCCAGAACAGCCAAAGCAATCCTGAGCAAAAAGAACAAGGCTGGAGGCTGGGCACAGTGACTCACGC11.3888888888888888No Hit
ATCCACCTGAAAGTTGCCAACCACCAAGAGGCCCGCAGCGTTGGTGCAGCCTGTGATGAGTGCCGTGGTGTTAAC11.3888888888888888No Hit
ACTTCTATACAAGAGACGCCAAAGTAAATAGGAAGGTGGATAACGCACTCCAAACGGCTAACTCCCAGGAGAGT11.3888888888888888No Hit
GTGTAGGAGGGTTTTGTTTCTCAGTTCCTCCTGACAGAGTAGTTAGCTGA11.3888888888888888No Hit
CTTCCTGGCTGTTCCGGAACCACCTGACTTCAATGCTGCCTGGATAGAAA11.3888888888888888No Hit
CCTGACAGCTGACCAAGGGGCAGAGCTGAGAAGAGGGCGTGGTAGTTCTG11.3888888888888888No Hit
GTATCAACGCAGAGTACGGGATCGCTTCTCGGCCTTTTGGCTAAGATCAAGT11.3888888888888888No Hit
GAGCCAAACTCTACTGCAAGGACAGTCAGAGTTTTAGAAGAGGCTACTTA11.3888888888888888No Hit
AGGCAGGTGGATCGCTTGAAGCCAGAAGTTCAGACCAGCCTGGCCAACAT11.3888888888888888No Hit
CAATCTCGTTCAGCTCATGATGCCCAATCATTGACAGACATGCCATCATAACATATGCATAACCAAAGGATATGT11.3888888888888888No Hit
CCCTTACCGGCAAGACCATCACCCTTGAGGTGGAGCCCAGTGACACCATCGAAAA11.3888888888888888No Hit
TTCAATCACTGATTAATACAATTTTACTCTGTCTCTATTTTACCCTCAAACAAGAATCAGAGTAATTCGAGTAT11.3888888888888888No Hit
ATGCGTGACTTACCACGCGAAGCAAATGTGTATCAAGTAGTAATCTTTCCAAAGAGTCAGTTTGCAGATCAGG11.3888888888888888No Hit
GAGGATGGGCGAGCAGTCTGAATGCCAGAATGGATAACCGTTTTGCTACAGCATTTGTAATTG11.3888888888888888No Hit
GGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTT11.3888888888888888No Hit
GTATCAACGCAGAGTACGGGGGGGAAGCGGCGGCAGGCGCCATGTCCGGCCGCGAAGGTGGCAAGAAGAAGCCA11.3888888888888888No Hit
ATCTTGTACTGGCGTGGATTCTGCATAATGGTGATCACACGTTCCACCTCATCCTCAGTGAGTTCTCCCGCCCT11.3888888888888888No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT11.3888888888888888No Hit
GGGATAACCCTACACCCAGACCCCAGGCTGGACATGCTGACTGTCCTCTC11.3888888888888888No Hit
GTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCA11.3888888888888888No Hit
GTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTGGAGCTACTATACTGCTGACAGTAATACA11.3888888888888888No Hit
ACATAAACAATATAATAAACGCAGACGAAAATTGAAAGAATAAATGAGAGTGGTAGGAAGAAAAAACTTTCTCTT11.3888888888888888No Hit
AAACTAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAA11.3888888888888888No Hit
CTGTATATTTAGTCACCACCTCAATCACTGCTAAGCTCTCTTCATGTCTATCTTTACCACAGTTTCCCATCTTAG11.3888888888888888No Hit
ACATAGGAGATGTCCAGCTGGATAAGCAGTGACACCCCTACTCCTGCCAGGCTGCCCACTCCTGCTGTGAACACA11.3888888888888888No Hit
ATTCTGTGGGACTCTGCTGATATCAAAGACGTAACTCTAAAGTTCCCACG11.3888888888888888No Hit
GCTGATACCAGGCCAACCAACTTCTTATACTCTCACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACTGATG11.3888888888888888No Hit
GCCATACCACAGTAACTAGATCTAATGTGAGGGCTAAATGCCTGGAGAGG11.3888888888888888No Hit
GTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATCACGTGCCTGGTGACGGG11.3888888888888888No Hit
GCAGAGGACAGTGGAGCAGCCAACACACAAAACTACCGTTTGTGCATGGC11.3888888888888888No Hit
CCACTCGATAAAGGTTAAGATAACAAACAATTGAAAGTTGTAATACTGGA11.3888888888888888No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGAAAAGGGGCTGGAGTGGGTTG11.3888888888888888No Hit
TTCCAGCTCCTCATCCATACCCTGATTTATAGCATCACGAACTGTCACCT11.3888888888888888No Hit
CTATAAAGGTTGTTGGGATGTTTTTTTGTTAAATACAGAGGGTCTTCCTC11.3888888888888888No Hit
ATCTTGTACTGGAGTGGATTCTGCATAATGGTGATCACACGTTCCACCTCATCCTAAGTGAGTTCTCCCGCCCT11.3888888888888888No Hit
CCATGAGTGAGGGGAGGAAAGGGGTGGGTATTGAATTTTCAAACTGTCATTATATTTTTACGTTGTTAGATAT11.3888888888888888No Hit
CTGACACCCTCCGCGAGTTTGTCACTGCTTTCAAAAAAGGAAAACTGAAGCCAGTCATCAAATCCCAGCCAGTGC11.3888888888888888No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT11.3888888888888888No Hit
GACCCAGGTGCAGGAAATGCTTAGAAGTTTGGTGGCTCACACAGGGGTATGTTTAGTGGGGAGATAACTGCTGAA11.3888888888888888No Hit
CTCTAGTTCAGAAGCTAATGCACAAGCAGAGCCAGACATGGCCGCCAACCAGCTGGCACTCCAACGGATA11.3888888888888888No Hit
GTCCACTCCACGCATCGACCTGGTGTTGCAGTACCTCCAGGAACGGTGCACTCCCTCCGGGGATACAACGTGTTT11.3888888888888888No Hit
GGCTCAGCCAGAGCAAGTTCACCTGCCCCGAGGATCACAGCGTCCACACATACCACCAGAATGCGTACTAAAA11.3888888888888888No Hit
CTCAGAGCCCTTGCCCGCTCGGGTATGAAGATCGGGCGGATTGAGGATGTCACCCCCATCCCCTCTGACAGCA11.3888888888888888No Hit
GTCTTGCTGGCTCAGGAGTCAAGCTGCAGACCTTCGTAGTGAATGTTACA11.3888888888888888No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTG11.3888888888888888No Hit
AACCCAGAGAATGCAGAAACCATGAAACCCACAAGAATGAAGAGCAGAAT11.3888888888888888No Hit
GTCGGCGTCCCCCAACTTCTTAGAGGGTCAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC11.3888888888888888No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA11.3888888888888888No Hit
ACCACAGAACCTATGCAAAGAGTGAGGAGAGTGAGCCAGAGAGGGGTCCAGGCCATGGTGGAGACACCCCAGAA11.3888888888888888No Hit
GCCGTAGGCAGCCATGGCGCCCAGCCGGAATGGCATGGTCTTGAAGCCCCACTTCCACAAGGACTGGCAGCGGCG11.3888888888888888No Hit
CAACCTGGTGCGCATTGCTATCCAGAACCTGCTGTACTACGGCGTTGTGACACTGGTGTCCATCCTCCAGTACTC11.3888888888888888No Hit
ACCATGATCGACGAGCCCACCCGCGGCGCCTTCCCGCCCTAGATCATGGAGACCTCCTCCACCAGTAGGCGCAG11.3888888888888888No Hit
GGGATGAGGTACAGGGTGGGAGATGGGGGAGGGGCTGGGGGCTGAAGGGAGCAAGAAAGCTGTAGCTGTGTGGGG11.3888888888888888No Hit
GGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGG11.3888888888888888No Hit
CTGAAACAGGAGGCGAAAACCTTTCTCGCGACATTGCCAAGCAGGAGCGA11.3888888888888888No Hit
CTCCTGGTCGGCCTTCGACTTCGGCTCGACGGCGATCTCAATGACGGGCTCCGGGAACTCCATCCGTTCCAGGAT11.3888888888888888No Hit
TTGGTATACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGGTCTTACGCGGAGGCTATTTC11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG50.057.025
CGGAGGC50.057.051
CAGTCCA50.057.015
TATACTC50.057.05
TGGTATA50.057.02
CCAGTCC50.057.014
CCTGTGT50.057.022
GGTCTTA50.057.042
TCGGTTT50.057.028
TACGGTC50.057.039
GGGTACG50.057.036
GTTTCGG50.057.031
GGTTTCG50.057.030
ACTCTAC50.057.08
TCTACCA50.057.010
GCTATTT50.057.056
GCGGAGG50.057.050
CTCTACC50.057.09
ACCTGTG50.057.021
GTATACT50.057.04
Produced by FastQC (version 0.11.5)