FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359584
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18640.13710076023254172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35850.040.1939541
GGTATCA15800.037.437531
TATCAAC41650.034.2538342
TCAACGC42000.033.8046844
ATCAACG42350.033.5253073
CAACGCA43350.032.9105455
AACGCAG43550.032.7618186
GTGGTAT6050.032.4007721
ACGCAGA46550.030.7401087
AGAGTAC47650.030.10930811
CGCAGAG48350.029.5299858
CAGAGTA49350.029.14182710
GCAGAGT51900.027.7100039
GAGTACG40200.027.21627612
AGTACGG39950.027.21434813
ACGGGTA1701.7462298E-1024.27728716
GTACGGG44500.024.12160714
CATACGG750.002034810622.9184285
TGGTATC7650.022.4690482
TACGGGG19200.022.040215
Produced by FastQC (version 0.11.5)