FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294104
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7370.25059162745151375No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6320.2148899708946495No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5420.1842885509887659No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5060.17204798302641242No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4900.1666077305986998No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT4440.15096700486902592No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG4340.14756684710170553No Hit
GTAGTATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGAAACA4340.14756684710170553No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC4010.13634632646954817No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG3960.134646247585888No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT3830.13022604248837144No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG3720.126485868944319No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC3580.12172564807007046No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT3550.12070560073987432No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC3540.12036558496314229No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT3450.11730544297255392No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3440.11696542719582187No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG3390.11526534831216169No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGCCACTCAGACTACTGTCATAGGA3380.11492533253542965No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG3240.11016511166118109No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG3180.10812501700078883No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC3150.10710496967059273No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3080.10472485923346843No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT3070.10438484345673639No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG3060.10404482768000436No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA3050.10370481190327231No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC3030.10302478034980823No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGCCACTCAGA2970.10098468568941599No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG2960.10064466991268396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC150.002392130468.719484
AACGATC252.3709095E-456.08157757
CTGATCG200.00747301751.5396113
CTGATAC200.00747301751.539614
GTATCAA4050.043.3046341
TATACAC803.6379788E-1142.9496773
TGATACA350.001373413339.268285
TATAAGA350.001373413339.268284
GGTATCA1600.038.6875951
TGGTATC451.0240212E-438.1774942
TACACAT1051.6370905E-1135.995925
TATCAAC5050.034.699942
CGTATGC1003.7471182E-1034.53588541
GTGGTAT400.00263451834.3889731
TCAACGC4950.034.0126724
ATCAACG5100.033.6860243
AGGGGGG1002.275325E-433.3175170
ATGCCGT1056.184564E-1032.9532644
TCGTATG1056.384653E-1032.8632440
CAACGCA5150.032.691795
Produced by FastQC (version 0.11.5)