FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134286
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51370.240689392143321No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG48680.22808564550392965No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT45750.2143574010231056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43470.20367467152949512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41300.19350733687987456No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39990.18736945282872117No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC38400.17991965462922965No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36500.17101738005122089No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36040.16886209252180823No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG35710.16731590798983828No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34860.16333331146809751No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33950.1590695904859986No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC33420.1565863244195014No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAA33020.15471216135044694No Hit
GTGTGTATATCTTCCATCAAAAGAGATGACTGCCACCCACTCCGGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG32940.15433732873663605No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32560.1525568738210343No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32020.15002675367781074No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATTACTGAACACAGA31920.14955821291054713No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31670.1483868609923881No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCAC31140.14590359492589092No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATG29440.13793840188240938No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT29220.13690761219442943No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA28650.1342369298210268No Hit
ATATACACACTATGCAGCCTCCGTGAAGGACCGCTTCACCATCTCCAGAG28440.13325299420977318No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27970.1310508526036342No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG27350.12814589984659974No Hit
AGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAACCAGGGAA26620.12472555224557534No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26450.12392903294122719No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26100.12228914025580451No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26050.1220548698721727No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT25980.12172689133508818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25420.11910306303841191No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC25240.11825968965733738No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25160.1178848570435265No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC24570.11512046651667115No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24560.11507361243994478No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24520.11488619613303935No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA24500.11479248797958662No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24460.11460507167268116No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCCGGAGTGGGTGGC24400.11432394721232299No Hit
GCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC24050.11268405452690033No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23990.11240293006654214No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23920.11207495152945762No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT23690.1109973077647513No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATGAGGGGGAGA23580.11048191292076133No Hit
CTATGCAGCCTCCGTGAAGGACCGCTTCACCATCTCCAGAGACAATTCCAGGAACACTCTGTATTTGGAAGTCAA23510.1101539343836768No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23120.10832662539134869No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG23070.10809235500771686No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22760.10663987862919964No Hit
CCCTAACACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGGGGCTATGCT22280.10439088294633427No Hit
TTACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC21610.10125165980566803No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21580.10111109757548895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT503.7973095E-641.47421339
GTGGTAT5900.037.9203831
ATCAACG50300.035.284813
TCAACGC50750.034.9041634
CGATTAA1101.2789524E-534.74533570
CAACGCA51200.034.664575
CGTATGC1600.034.6050241
TATACCG400.002635616634.395855
AACGCAG51750.034.3626176
TCGTATG1550.033.4658540
AGAGTAC55200.032.0287711
CGCAGAG56050.031.8491468
CAGAGTA57000.031.31832910
GCAGAGT59850.029.8844499
ACGCAGA61650.028.8996497
CTTATAC2700.028.0459631
AGTACGG50500.027.10865413
GAGTACG50550.027.01379412
ACGAGAC2550.026.98282622
TGTTAGA13550.026.32610770
Produced by FastQC (version 0.11.5)