FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534512
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGA75470.49181759412764453No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGA68160.44418029966530076No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGC66700.4346658742323292No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55990.36487169862470936No Hit
GGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGG54400.3545100983244185No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC49650.32355563201851795No Hit
GTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG46660.30407061007017216No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46540.30328860250033884No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44940.2928618349025619No Hit
GTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC42970.2800238772977989No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42210.27507116268885484No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39020.2542827947907869No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38580.25141543370139824No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35910.23401576527260784No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35700.23264725202539963No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34880.22730353363153888No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT32570.2122498879122483No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32290.21042520358263736No Hit
GTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG31660.20631966384101266No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG31530.20547248897369325No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30890.20130178193458245No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAG30680.19993326868737424No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29150.18996267217199994No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28950.18865932622227785No Hit
TCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGG28070.18292460404350047No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGAC28010.18253360025858384No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27640.1801224102515979No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC27570.17966623916919516No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27190.17718988186472312No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27130.17679887807980646No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG25900.1687833004890154No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25380.16539460101973785No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25280.1647429280448768No Hit
GTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCC24510.15972504613844662No Hit
GTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG24290.1582913655937523No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCT24260.15809586370129397No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGG23810.15516333531441917No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA23750.15477233152950254No Hit
GCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCC23560.15353415287726652No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT23520.15327348368732208No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT23510.15320831638983598No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC23490.1530779817948638No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23010.14994995151553067No Hit
TCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTCAGGGACC22940.14949378043312794No Hit
GTCTGGCGCCTCAGCCTCCCTGGCCGTCACTGGGCTTCAGGCTGAGGATG22720.14806009988843358No Hit
GTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAG22640.14753876150854472No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22640.14753876150854472No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22620.14740842691357253No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTA22610.1473432596160864No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22450.1463005828563087No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22390.1459095790713921No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21960.1431073852794895No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT21270.13861084175294816No Hit
GCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCT21230.13835017256300375No Hit
GGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAG21200.13815467067054543No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC21100.13750299769568436No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21070.13730749580322604No Hit
GATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGG20730.1350918076886984No Hit
CTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGG20660.13463563660629568No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20610.13430980011886515No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT20410.13300645416914303No Hit
GCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCC20340.13255028308674027No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC20130.13118176983953203No Hit
GTCAGGGACCCCTGACGGCCGATTGTTGTTACCATAGATGACGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA19800.12903124902249055No Hit
CCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGG19710.12844474334511558No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19640.12798857226271285No Hit
GCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCA19550.12740206658533787No Hit
AGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGG19310.12583805144567134No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG19080.12433920360349088No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18930.1233616941411993No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18880.12303585765376876No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTC18740.12212351548896326No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18660.12160217710907441No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18560.12095050413421335No Hit
GGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC18430.12010332926689396No Hit
GTCCCTGACCGCTTCTCTGGCTCCCAGTCTGGCGCCTCAGCCTCCCTGGC17480.11391243600571387No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17360.11313042843588059No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17270.11254392275850562No Hit
GGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGG17260.11247875546101954No Hit
ATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTA17180.11195741708113069No Hit
GGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCT17100.11143607870124184No Hit
TCACTGGGCTTCAGGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTAT17070.11124057680878349No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16980.11065407113140856No Hit
GCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAG16700.10882938680179757No Hit
GCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGGCTCCCAGT16580.10804737923196431No Hit
GGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGAC16470.10733053895961714No Hit
GTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTC16450.10720020436464492No Hit
CCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTC16370.10667886598475607No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16320.10635302949732553No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16290.10615752760486721No Hit
CCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGC16120.1050496835476034No Hit
GTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGC15970.1040721740853118No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15940.1038766721928535No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15860.10335533381296465No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG15830.10315983192050633No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCA15620.10179131867329809No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG15470.10081380921100651No Hit
GTTCCTGGAAGCTGCTGGTACCAGTGGACATCATAACCTGCCCCGATGTT15430.10055314002106208No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG15360.10009696893865931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAC200.00704604252.32279260
GTGGTAT3200.045.300191
GGTATCA11900.045.245811
GTATCAA29200.041.842841
TGGTATC3850.038.5184362
TATCAAC33350.036.6073532
TCAACGC35100.034.978714
ATCAACG35200.034.7813643
CAACGCA35900.034.2953075
AACGCAG36500.033.8260356
TAAACCG553.264497E-431.3522155
CGCAGAG39500.031.169668
GTAGTTT2459.822543E-1131.00468370
ACGCAGA39850.030.9804237
AGAGTAC39800.030.93471111
CAGAGTA40100.030.78928410
GCAGAGT43300.028.6731599
AGTACGG36450.027.43856413
GAGTACG36700.027.25165412
TACGGGG19150.025.03184115
Produced by FastQC (version 0.11.5)