FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534512
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGA74220.4836716819418812No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGA61240.3990845298049152No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGC61040.39778118385519307No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54710.3565302845464878No Hit
GGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGG51030.3325487190716006No Hit
GTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG48700.31736473875733784No Hit
GTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC48390.31534455253526855No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC46080.300290906815978No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45300.2952078576120617No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42600.27761268729081295No Hit
GTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG38960.2538917910058703No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37960.2473750612572596No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36810.2398808220463574No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36790.2397504874513852No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34930.22762937011896944No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33720.21974412712315056No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31570.20573315816363769No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAG31200.20332196815665174No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG31090.2026051278843046No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30150.1964794019206106No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC30080.19602323083820786No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29240.19054917784937492No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT28540.18598746702534746No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27820.18129542160634782No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27620.1799920756566257No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27270.17771122024461197No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27220.17738538375718144No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGAC26630.17354051320550118No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG26370.1718461634708624No Hit
TCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGG26270.17119449049600133No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25810.16819679481164046No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25120.1637002512850991No Hit
GTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG24930.16246207263286308No Hit
GTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCC24520.15979021343593272No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24320.1584868674862106No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC24140.1573138561314607No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23190.15112296287028057No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC22840.14884210745826687No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA22630.14747359421105863No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGG22350.14564890988144766No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCT22230.1448669023116144No Hit
GCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCC22090.14395456014680888No Hit
TCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTCAGGGACC22020.14349838906440615No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT21740.14167370473479518No Hit
GTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAG21600.14076136256998967No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21530.14030519148758694No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT21510.14017485689261472No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT21250.13848050715797594No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20940.13646032093590665No Hit
GGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAG20680.1347659712012679No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20570.1340491309289207No Hit
GTCTGGCGCCTCAGCCTCCCTGGCCGTCACTGGGCTTCAGGCTGAGGATG20510.1336581271440041No Hit
AGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGG20410.13300645416914303No Hit
GCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCC20380.1328109522766847No Hit
GCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCT20290.13222444659930974No Hit
GATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGG20250.13196377740936532No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20190.1315727736244487No Hit
GTCAGGGACCCCTGACGGCCGATTGTTGTTACCATAGATGACGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA20040.1305952641621571No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20030.13053009686467099No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20010.13039976226969877No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19990.13026942767472655No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC19950.13000875848478213No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19110.1245347054959492No Hit
GGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC19100.12446953819846308No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT18970.12362236333114372No Hit
GGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGAC18660.12160217710907441No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTC18460.12029883115935228No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18380.11977749277946342No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18300.11925615439957457No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTA18100.11795280844985247No Hit
CCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGG18050.11762697196242193No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17500.11404277060068609No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC17240.11234842086604731No Hit
GCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAG17040.11104507491632519No Hit
GCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCA16950.11045856923895023No Hit
GTCCCTGACCGCTTCTCTGGCTCCCAGTCTGGCGCCTCAGCCTCCCTGGC16910.1101979000490058No Hit
GTTCCTGGAAGCTGCTGGTACCAGTGGACATCATAACCTGCCCCGATGTT16760.1092203905867142No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16620.10830804842190873No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16420.1070047024721866No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16270.10602719300989501No Hit
GTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTC16200.10557102192749225No Hit
TCACTGGGCTTCAGGCTGAGGATGAGGCTGATTATTCCTGCCAGTCCTAT16170.10537552003503393No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16130.10511485084508951No Hit
GGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGG15980.10413734138279793No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15930.10381150489536739No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15830.10315983192050633No Hit
CCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGC15620.10179131867329809No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCA15550.10133514759089535No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15520.10113964569843702No Hit
GTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGC15490.1009441438059787No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG15420.10048797272357596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4500.046.7163241
GGTATCA13350.042.5945471
TGGTATC5000.042.032362
GTATCAA31500.039.823751
TATCAAC36050.034.7872472
ATCAACG37400.033.25523
TCAACGC37900.033.0891884
CAACGCA38900.032.3271335
AACGCAG39500.032.013666
ATATGTC450.00465426430.624673
AGAGTAC41900.030.36123311
CGCAGAG41800.030.2669418
CAGAGTA42200.030.14539310
ACGCAGA42900.029.407637
GCAGAGT45300.028.2346739
ACGCGAC500.007595116727.70392843
GAGTACG40850.026.2467412
AGTACGG40750.026.2265513
TACGGGG23250.025.50341815
TTAACGG1501.029548E-925.32890335
Produced by FastQC (version 0.11.5)