FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865776
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55940.29982162917735034No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCT55830.2992320621553713No Hit
CTTTTATACAGCTCCAACAGTAAGAATACCTTAGCTTGGTACCAGCAGAA54380.2914604968656473No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGGTATTCTTACTGTTGGAGCTGTATAAAAGACT49810.26696666695251736No Hit
ACATTATAGTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT42350.22698330346193757No Hit
CTATAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC41720.22360669233605748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41020.21985490219619078No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37410.200506384474878No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36820.19734416135699034No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTG35550.19053734210323212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33780.18105067274956907No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33670.18046110572759003No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31790.17038486935194794No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA31750.17017048134395554No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA28990.155377708792481No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA27850.149267650564698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26930.14433672638087316No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26900.14417593537487886No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCTCGGGT26400.14149608527497406No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26240.14063853324300452No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25720.13785148913910353No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23660.1268105067274957No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23650.12675690972549758No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23510.12600655169752425No Hit
GTACTATAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT23400.12541698467554518No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22570.12096843350970321No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC22340.11973570246374698No Hit
TTATAGTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTT22010.1179670013978098No Hit
GTTTATTACTGTCAGCAACATTATAGTACTCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAA21620.11587671831988407No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21540.11544794230389929No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTCACTACTGGGGCTGGATCCG21530.1153943453019012No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20920.11212492818001732No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAA20760.11126737614804778No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC20610.11046342111807633No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20580.11030263011208205No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20540.11008824210408967No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20450.1096058690861068No Hit
GAGTACTATAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT20420.10944507808011252No Hit
GGGATATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGT20350.10906989906612582No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19770.10596127295023625No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19310.10349581085832382No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19130.10253106482235809No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19000.10183430379638284No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18710.10027999073843805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6250.039.0913931
GGTATCA16050.039.021071
GTATCAA35550.038.2349321
TGGTATC6550.035.718152
TATCAAC40350.033.680222
ATCAACG41650.032.7115823
TCAACGC42000.032.4389844
CAACGCA42400.032.2952425
AACGCAG43550.031.4466576
AGAGTAC47750.028.62548411
CGCAGAG48400.028.3099128
CAGAGTA48900.028.1627610
GCAGAGT50350.027.487749
ACGCAGA49850.027.4827677
TTAACGG1208.519055E-825.87655435
TAACGGC1251.2617238E-724.847536
AGTACGG45950.024.12715313
GAGTACG46950.023.61326212
TGTTAGA12150.022.8663670
CTGTATA19900.021.80644660
Produced by FastQC (version 0.11.5)