FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498231
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAA405.1887244E-542.8520517
GTATCAA18800.039.9585271
GGTATCA7950.036.243941
ACGATTA400.002542700434.6432740
TATCAAC21850.034.5135232
ACGGGTA807.4869604E-834.28164316
TCGACTG400.002678306534.2782025
ATCAACG22900.032.9310263
TCAACGC23050.032.716724
AACGCAG23300.032.5128026
CAACGCA23250.032.4352875
ACCGAAC450.004487132730.85034242
ACGCAGA24750.030.6080137
AGAGTAC26100.029.15909811
CAGAGTA26450.028.77324910
CGCAGAG26700.028.5009778
TACGTGA500.00714701828.04746653
AGTACGG20000.027.93953713
GCAGAGT27600.027.5715969
GAGTACG20500.027.42531412
Produced by FastQC (version 0.11.5)