FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345622
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24850.18467296164896232No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22980.17077604260334625No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17990.1336928201233333No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG15900.1181609694252918No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15710.11674898299819712No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15240.11325617446801553No Hit
GTCTGGGACAGACTTCACTCTCGCCATCAGCAGCCTAGAGCCTGACGATT14350.1066421327832036No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGGGGCCCTGTT13800.10255480365214005No Hit
GCCTAGAGCCTGACGATTTTGCAGTTTATTACTGTCAGCAACGTAGCAACTGGCCTCACACTTTTGGCCAGGGGA13730.10203459812636834No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13650.10144007752548635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATT350.00518402656.5400970
GGTATCA16550.045.1431661
GTATCAA40400.039.4349631
TCGTATG1650.035.5133440
TATCAAC45900.034.978322
TCGACGT2350.034.89409629
TCAACGC47250.033.9068034
ATCAACG47300.033.870963
CAACGCA48500.033.2437635
CGACGTC2500.032.81878730
CTCGACG2400.032.72400728
CGTATGC1900.032.69399641
AACGCAG49550.032.6080976
ACTCGAC2600.031.51190427
CGCAGAG54800.029.4852398
AGAGTAC54800.029.42303511
GACGTCA3100.028.68836431
AGTACGG40950.028.46945213
CTTATAC3000.028.4292011
ACGCAGA57100.028.3562167
Produced by FastQC (version 0.11.5)