FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717574
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCAGTCACAAAGGTCAACTCCAGGGAGA25730.35856929041464713No Hit
GTCTCTGGTGACTCCATCAGCAGTAGTGATCACTACTGGAGTTGGATCCG22760.31717983093032914No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21700.30240783528946147No Hit
CTACTGGAGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGTTACATCGATTACAGTGGGATCAC18650.2599035082096063No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17290.2409507590854741No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15650.21809597337696182No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15410.21475137059035027No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14870.20722601432047427No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14820.20652922207326352No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13180.18367443636475123No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11900.16583655483615628No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11900.16583655483615628No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11680.162770668948429No Hit
GTAGTAGGTGATCCCACTGTAATCGATGTAACCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAACT11560.16109836755512325No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11380.15858991546516457No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10670.14869546555477203No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10630.14813803175700346No Hit
GTCTATTACTGTGCCAGACTCTTCGGGTATACCAGTGGCTGGTACGTCGA10200.14214561843099108No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10190.14200625998154892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9930.13838294029605308No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9810.13671063890274732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9740.13573512975665228No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9740.13573512975665228No Hit
CACGTGCACTGTCTCTGGTGACTCCATCAGCAGTAGTGATCACTACTGGA9690.13503833750944153No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9650.13448090371167293No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9620.1340628283633465No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9110.12695554744179693No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9080.1265374720934705No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACAC9000.12542260449793333No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8970.12500452914960689No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8970.12500452914960689No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8960.12486517070016473No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8910.12416837845295398No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8880.12375030310462753No Hit
GGTATACCCGAAGAGTCTGGCACAGTAATAGACGGCCGTGTCTGCGGCAGTCACAAAGGTCAACTCCAGGGAGAA8870.12361094465518539No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA8850.1233322277563011No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8810.12277479395853251No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8750.12193864326187961No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8710.12138120946411102No Hit
CTCCAGTAGTGATCACTACTGCTGATGGAGTCACCAGAGACAGTGCACGT8660.12068441721690026No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8620.12012698341913168No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8250.1149707207897722No Hit
CTGTAAAGTGGAAGCACCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT8210.11441328699200362No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT8170.11385585319423502No Hit
CAGCAGTAGTGATCACTACTGGAGTTGGATCCGCCAGCCCCCAGGGAAGG8160.11371649474479287No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8150.11357713629535071No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8040.11204419335148709No Hit
GTCTGGCACAGTAATAGACGGCCGTGTCTGCGGCAGTCACAAAGGTCAAC7980.11120804265483421No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7980.11120804265483421No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACGTGCA7830.10911766591320199No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA7780.10842087366599125No Hit
GGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACGTGCACTGTCTCTGGTGACTCCATCAGCAGTAGTGATCA7760.10814215676710694No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT7760.10814215676710694No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGATCCAGTTCTCCCTGGAGTTGA7740.10786343986822265No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7730.1077240814187805No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG7670.10688793072212763No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA7630.10633049692435903No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT7570.10549434622770612No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7510.10465819553105325No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7470.10410076173328466No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC7340.10228910189053672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)