FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009037
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53750.26754111546974996No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT50090.24932343207218186No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50010.24892523134218036No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46160.22976182121085872No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC43990.21896062640956834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43550.21677052239456018No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41140.20477472540326533No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG40590.20203709538450512No Hit
GTGCAGCCACAGTTCGTTTGATATCGACTTTGGTCCCAGGGCCGAAAGTG39780.19800531299324006No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39680.1975075620807382No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37880.1885480456557047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37430.18630816654944632No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37340.18586019072819465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37330.18581041563694448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35390.17615404793440836No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT34450.17147518935689088No Hit
TCTATGCCCCGTCCCTGAAGACACGAGCCACCATATCCGTAGACACGTCC34380.1711267637181396No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA33130.16490487731186632No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA32930.1639093754868626No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC32470.16161972128935403No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32460.16156994619810386No Hit
GAAATACACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGCCTCAGGGAAA31980.15918074181809494No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCGACTTTGGTCC30720.1529090803205715No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30280.1507189763055633No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30020.14942482393305848No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA29390.14628899318429675No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTAATCGGGCC29170.14519394117679266No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA29060.1446464151730406No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT29000.1443477646255395No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA28940.1440491140780384No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28740.14305361225303465No Hit
GCATTATAGTGGGAGCGCCTTCTATGCCCCGTCCCTGAAGACACGAGCCACCATATCCGTAGACACGTCCAAGAA28430.1415105844242789No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27860.13867340422301827No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT27740.13807610312801608No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26900.13389499546300043No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC26820.13349679473299894No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA26630.1325510679992454No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG26310.13095826507923944No Hit
CTCCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT26050.12966411270673464No Hit
CTATAATGCATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG25750.12817085996922903No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA25490.1268767075967242No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25450.12667760723172344No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25390.12637895668422236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25230.12558255522421938No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG25140.12513457940296768No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCA24760.1232431259354606No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCCATATTCACTTTCGGCCCTGGGACCAAAGTCGATATCAAACGAA24560.12224762411045689No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG24480.12184942338045542No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23340.1161750629779342No Hit
GTGTATTTCTGTGCGAGACGGCCTACCAGCAACAGCTACTACGAGTACTT23060.114781360422929No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA22860.11378585859792528No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22840.1136863084154249No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22780.11338765786792378No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA22710.11303923222917248No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22300.11099845348791486No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22210.11055047766666318No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22080.10990340148041076No Hit
CTCCACAACTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCCCT22070.10985362638916059No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG22010.10955497584165946No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21900.10900744983790743No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC21730.10816127328665424No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21660.10781284764790294No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT21460.10681734582289923No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA21450.10676757073164904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21440.10671779564039886No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21350.10626981981914718No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG21290.10597116927164607No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC21050.10477656708164161No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT21020.10462724180789104No Hit
GGATTGGGAGTATGCATTATAGTGGGAGCGCCTTCTATGCCCCGTCCCTG20760.1033330894353862No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC20720.10313398907038547No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT20580.10243713779288287No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG20570.10238736270163266No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20510.10208871215413155No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA20510.10208871215413155No Hit
CTACAAACTCCCATATTCACTTTCGGCCCTGGGACCAAAGTCGATATCAAACGAACTGTGGCTGCACCAT20500.10203893706288136No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCATTTGCAATGTCTCTGGTGCCTCCATCAGCAATAGTGCTTA20430.10169051142413008No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG20100.10004793341287394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4000.046.590862
GGTATCA12800.046.1330951
GTGGTAT4200.045.2209661
CTAGTGC350.001345804439.4419983
GTATCAA29550.037.7460481
TAGCGCG400.002592463734.5117454
TTGTCGC400.002592780234.5108879
TATCAAC35400.031.4895322
ATCAACG35700.031.1282423
TCAACGC36050.030.8260294
TGTTAGA11750.030.78827370
TTTGTCG450.00461637430.6763468
AACGCAG37050.030.2734646
CAACGCA37200.030.0586195
CGGTAGG703.827855E-529.5991781
GTAGTTT2502.6921043E-1028.42417370
CGCAGAG39350.028.4155758
AGAGCAT8100.027.91375270
TCGATTC500.0076673127.65068837
CAGAGTA40100.027.62592310
Produced by FastQC (version 0.11.5)