FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039579
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22220.21374036989973824No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAAATA19440.18699877546583762No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19380.18642161875143687No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16480.15852571088873477No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16060.15448561388792964No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14960.14390440745724953No Hit
GTATTATTGTGTGAGAGAGATTCATGGGTATGATTACGTTTGGGAAAACTATCGTTATAGGAGCTACTACTTTGA14640.14082623831377894No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG14420.13870999702764292No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14100.13563182788417233No Hit
CTCCAGCCCCTTCCCTGGAGCCTGCCGGACCCAGCTCATCCAATAGCTGC13460.12947548959723118No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13420.12909071845429734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12540.12062575330975328No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11880.1142770294513452No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11810.11360367995121101No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11520.1108140891649408No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11280.10850546230733787No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11240.10812069116440405No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11030.1061006426640015No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10950.10533110037813384No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10890.1047539436637331No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG10850.10436917252079929No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10760.1035034374491982No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10670.10263770237759708No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10670.10263770237759708No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10620.10215673844892982No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10560.10157958173452908No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC10530.1012910033773287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.038.4541361
GTGGTAT2850.032.7197461
TATCAAC17250.032.199352
TACGCAT553.1484803E-431.5450749
ATCAACG17950.030.7514673
ACCGTAC450.0046120830.68080327
TCAACGC18200.030.3290584
CAACGCA18400.029.9993955
AACGCAG18750.029.4394076
AGAGTAC19750.027.9514911
CCCTATA500.007735347427.5994422
ATACCGC500.007735347427.5994427
CAGAGTA20500.027.4337910
GTAGACA5200.026.90747670
CGCAGAG21600.025.714768
ATGTGTA1359.416908E-925.55749911
TATACAC951.05080635E-525.4205383
TTACATA700.001240636724.95077759
CCGACTA700.001330183824.65421527
TGTATAA1401.3864337E-824.6447314
Produced by FastQC (version 0.11.5)