FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039579
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23670.2276883238310893No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22400.21547184004294043No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAAATA17540.16872214617648107No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17540.16872214617648107No Hit
GTATTATTGTGTGAGAGAGATTCATGGGTATGATTACGTTTGGGAAAACTATCGTTATAGGAGCTACTACTTTGA16080.15467799945939653No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14450.13899857538484328No Hit
CTCCAGCCCCTTCCCTGGAGCCTGCCGGACCCAGCTCATCCAATAGCTGC13800.1327460443121687No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13680.13159173088336723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12330.11860570480935072No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11610.1116798242365419No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11590.11148743866507499No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11510.11071789637920736No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG11060.10638922102120185No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10820.10408059416359891No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10720.10311866630626436No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10670.10263770237759708No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10610.10206054566319635No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10600.10196435287746289No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10530.1012910033773287No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10520.10119481059159526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3250.040.2715261
GTATCAA15450.033.216641
TTCGTAG800.003607258232.2578870
TACGCCA1053.6743248E-430.72179470
TTCGGGG7450.030.30942270
TATCAAC17850.028.360532
GGTATCA11550.027.7331261
ATCAACG18400.027.6999573
CAACGCA18850.027.0386855
TCAACGC18850.027.0386854
AACGCAG19400.026.2784426
TATTAGA1251.286553E-724.7952062
AGAGTAC20700.024.3128411
CGCAGAG22000.023.1895458
CAGAGTA21750.023.13911610
GCCCATA1052.3099718E-522.9618381
GCGTAGA7650.022.77027370
GCAGAGT23350.021.996459
GTTATAG6550.021.79761354
TTAACGG800.00287862821.58903335
Produced by FastQC (version 0.11.5)