FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950614
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCGCGGCAGTCACAGAGTTCAGCTCCAGGGAGA29950.31505952994590863No Hit
GTCTCTGGTGGCTCCATCGACAGTGGTCTTTTCTACTGGAATTGGATCCG28250.29717635128453823No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23540.24762942687568246No Hit
GTGGATGCACCATAAATCAGGAGCTGAGGAGCATTCCCTGGTTTGTGCTGATACCAGGCTAAATAATTGCTAATG23060.2425800587830602No Hit
GTGCATCCACTGTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGA21930.23069300473167867No Hit
GTGTATTACTGTGCGAGATGCGGCCTGGGGGTGGCTTCCCCTCATATTGA17930.18861493729316No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17790.18714220493281183No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17180.18072529964843775No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16550.17409800402687106No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16450.17304605234090809No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16260.17104734413757847No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15060.15842392390602286No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14210.1494823345753376No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13690.1440121858083302No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13560.14264464861657836No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAAATGGGTC13510.14211867277359685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13480.14180308726780796No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13460.14159269693061535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13210.13896281771570795No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13200.13885762254711165No Hit
GTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTG12340.12981083804783014No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12210.1284433008560783No Hit
GTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC12050.12676017815853755No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT11940.12560303130397826No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAAACAGAGTCACCATCACTT11390.11981729703118196No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11340.11929132118820046No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11150.11729261298487083No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11130.11708222264767824No Hit
CAGTAATACACGGCCGCGTCCGCGGCAGTCACAGAGTTCAGCTCCAGGGA11090.11666144197329306No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10760.11319000140961526No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10470.11013934152032266No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC10440.10982375601453376No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10380.10919258500295599No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10300.10835102365418561No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10290.10824582848558931No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9980.10498477825910413No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT9840.10351204589875596No Hit
CCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGG9800.10309126522437077No Hit
GTATGACAGTGTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9740.10246009421279301No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9580.10077697151525224No Hit
GTCCAAGGGACACTGTCATACTTTTGACAGTAATAAGTTGCAACATCTTC9540.10035619084086705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT300.002937391665.257470
GTATAGG200.00731218451.8329621
CGCAGAT10000.041.11216470
GTATCAA12900.038.5733681
GTAGTTT1454.8647053E-836.00408670
TTAGAGT400.00258245134.5371324
TATCAAC14800.033.60372
ATCAACG15100.032.707353
GTGGTAT2450.032.439681
TCAACGC15400.031.845934
GCTATAC553.2361283E-431.3973963
CAACGCA15700.031.2374085
CTGGAGT12700.030.83026970
GGTTACC450.00457913330.72553332
AACGCAG16200.030.4864856
AATGGTA1502.5753216E-630.45345770
AGAGTAC17100.028.8849711
CAGAGTA17200.028.71703310
CGCAGAG17600.028.2576568
CCGCAGA9900.027.64948769
Produced by FastQC (version 0.11.5)