FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950614
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCGCGGCAGTCACAGAGTTCAGCTCCAGGGAGA27540.2897074943142011No Hit
GTCTCTGGTGGCTCCATCGACAGTGGTCTTTTCTACTGGAATTGGATCCG27060.2846581262215789No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23350.2456307186723528No Hit
GTGGATGCACCATAAATCAGGAGCTGAGGAGCATTCCCTGGTTTGTGCTGATACCAGGCTAAATAATTGCTAATG21300.22406570911011198No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20200.21249424056451935No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20160.21207345989013418No Hit
GTGCATCCACTGTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGA20100.21144228887855637No Hit
GTGTATTACTGTGCGAGATGCGGCCTGGGGGTGGCTTCCCCTCATATTGA16590.17451878470125623No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15270.16063302244654507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14990.15768755772584878No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14920.1569511915456747No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAAATGGGTC14720.15484728817374876No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14150.14885116356375985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13540.14243425827938574No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12930.13601735299501164No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12890.13559657232062647No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12640.13296669310571904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12440.13086278973379312No Hit
GTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC12170.12802252018169308No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11390.11981729703118196No Hit
GTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTG11340.11929132118820046No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11330.11918612601960418No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT11270.11855495500802639No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC11090.11666144197329306No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11040.11613546613031156No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11010.11581988062452268No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10970.11539909995013749No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAAACAGAGTCACCATCACTT10960.1152939047815412No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10930.11497831927575229No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10170.10698348646243376No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10110.10635231545085598No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT10040.10561594927068191No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9930.10445880241612264No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9880.10393282657314114No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9820.10330165556156337No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9600.10098736185244483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)