FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038896
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25610.24651168163127013No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAACTCAACTTCAGGGAGA22340.21503596125117433No Hit
GTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGT19480.1875067379217939No Hit
GTCTCTGGTGGCTCCATCAGCAATAATGGTGATTACTATTGGAGTTGGAT18680.17980625587161755No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18130.17451217446212133No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17600.1694106051038795No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17260.16613790023255456No Hit
GATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA16810.16180637907933038No Hit
GAGTTACAGTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT16610.15988125856678628No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16170.15564599343918928No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16070.15468343318291725No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15880.15285456869600036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15860.15266205664474597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15560.14977437587592984No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15560.14977437587592984No Hit
GTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGA15030.14467280651768802No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14330.1379348847237837No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14310.1377423726725293No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13850.13331459549367788No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13660.13148573100676103No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13610.131004450878625No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13310.12811677010980888No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAATAATGGTGATTACTATT12960.12474780921285672No Hit
GACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGTCCC12640.12166761639278618No Hit
ACTTACTACTGTCAACAGAGTTACAGTCCCCTCCCGCTCACTTTCGGCGG12460.1199350079314965No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11820.11377462229135545No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11820.11377462229135545No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11620.11184950177881137No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11470.11040566139440329No Hit
CCATAGATCAGGAGCCTAGGGGCTTTACCTGGTTTCTGCTGATACCAATT11420.10992438126626726No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11410.10982812524064006No Hit
GGAGGGGACTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGT11340.10915433306124964No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11230.10809551677935039No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTC11080.10665167639494233No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10860.10453404383114383No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10630.10232015524171813No Hit
GTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA10620.10222389921609092No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAA10490.10097257088293728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10480.10087631485731006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10430.10039503472917405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)