FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340262
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT52900.39469894692231816No Hit
GTAATACATAGCCGTGTCGTCAGGTCTCAGGCCATTCATCTGCAGATACA48620.3627648922374879No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46640.34799166133188886No Hit
ATGTATTACTGTGCGAAAGATCCGGGGCTCGTAGGTGGTCAGGGGTCCTGGCTGGTACGCCTTGACTCCTGGGGC42440.3166545048654666No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36860.2750208541315056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36250.2704695052161443No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT35590.26554509491427797No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33240.24801120974854168No Hit
GACCAGGGAGAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAA31340.2338348770613507No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30540.22786589487727027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30370.22659748616315317No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA29840.22264303546619987No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA29780.22219536180239385No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT29660.22130001447478181No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28080.20951127466122296No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT27160.20264694514953047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26470.19749869801576111No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25900.1932457982096038No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25720.1919027772181857No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCAAGCCATAGGTCC25080.18712759147092134No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC24260.1810093847322389No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24200.18056171106843288No Hit
GTCCCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTAGCA23940.17862179185860674No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23350.17421966749784742No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC23340.17414505522054644No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG22860.17056366591009817No Hit
GACTGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT22770.16989215541438912No Hit
GAGTTACAGTACCAGTCTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAGACGAACTGTGGCTGCACCAT22670.1691460326413791No Hit
GTGCAGCCACAGTTCGTCTGAGATCCACTTTGGTCCCAGGGCCGAAAGTG22660.16907142036407807No Hit
CAGTAATACATAGCCGTGTCGTCAGGTCTCAGGCCATTCATCTGCAGATA22070.16466929600331875No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG21720.16205786629778357No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT21240.15847647698733533No Hit
CCATAGATCAGGAGCTTAGGGGCTCTCCCTGGTCTCTGCTGATACCAATT20820.1553427613406931No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20410.15228365797135188No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC20360.15191059658484685No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20310.15153753519834182No Hit
GTACCAGTCTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAGACGA20220.15086602470263277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20070.1497468405431177No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20000.14922455460201065No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTAGCACTTATTTAAATTGGTATCAGCAGAGA19990.14914994232470966No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCAT19750.14735924766948552No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCCGGACCTATGGCTTGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG19500.1454939407369604No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGAGCAGCACTGAACACAGA19120.1426586741995222No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19030.14198716370381312No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA19010.14183793914921114No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18840.14056953043509401No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18560.1384803866706659No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18560.1384803866706659No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18480.13788348845225784No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAGATCCACTTTGGTCC18170.13557050785592667No Hit
GAATAAGTACTATTCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAACTCCGGGAACACCCTGTAT18170.13557050785592667No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18040.1346005482510136No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17290.1290046274534382No Hit
GTGCCAGATGTGGCATCCAGATGACCCAGTCCCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA17270.1288554028988362No Hit
GTTACAGTACCAGTCTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAGACGAACTGTGGCTGCACCAT16970.12661703457980603No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16840.12564707497489297No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC16810.12542323814298995No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16800.12534862586568896No Hit
GGGCTGGAGTGGGTGGCACTTATATCAAATGATGGAAGGAATAAGTACTA16730.1248263399245819No Hit
CTCCCTGGTCTCTGCTGATACCAATTTAAATAAGTGCTAATCCTCTGACTTGCCCGGCAAGTGATGGTGACT16700.1246025030926789No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA16530.12333409437856181No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA16400.12236413477364873No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC16360.12206568566444471No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16280.12146878744603666No Hit
GTATTACTGTGCGAAAGATCCGGGGCTCGTAGGTGGTCAGGGGTCCTGGC16250.12124495061413365No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCCAGATGACCCAGTC16150.1204988278411236No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16090.12005115417731756No Hit
GAGCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG15750.11751433674908338No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCAGTCTATTCACTTTCGGCCC15490.11557441753925725No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15450.11527596843005322No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15360.11460445793434418No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA15120.11281376327912004No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG15100.11266453872451805No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15020.11206764050611No Hit
GTACTATTCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAACT15000.11191841595150798No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG14900.11117229317849793No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGT14660.1093815985232738No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14620.10908314941406978No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT14600.10893392485946778No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT14590.10885931258216676No Hit
GTGAATAGACTGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATC14400.10744167931344767No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14340.10699400564964164No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA14330.10691939337234063No Hit
GCAGAGACCAGGGAGAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT14330.10691939337234063No Hit
GAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCAC14280.1065463319858356No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14220.10609865832202957No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14200.10594943376742758No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14130.10542714782632051No Hit
GTACTTATTCCTTCCATCATTTGATATAAGTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG14070.10497947416251449No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT13950.10408412683490242No Hit
GTCTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13940.10400951455760143No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCGAAGTTG13930.10393490228030042No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13870.10348722861649438No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13860.1034126163391934No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG13720.10236804445697929No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG13710.1022934321796783No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA13630.10169653396127026No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13590.10139808485206624No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13550.10109963574286222No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAG13430.10020428841525016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)