FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340262
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48480.36172032035527385No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT45140.33679981973673806No Hit
GTAATACATAGCCGTGTCGTCAGGTCTCAGGCCATTCATCTGCAGATACA42090.3140430751599314No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT40030.2986729460359243No Hit
ATGTATTACTGTGCGAAAGATCCGGGGCTCGTAGGTGGTCAGGGGTCCTGGCTGGTACGCCTTGACTCCTGGGGC39570.2952407812800781No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36250.2704695052161443No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34420.2568154584700604No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31220.23293952973373863No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31160.2324918560699326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29270.21839013566004262No Hit
GACCAGGGAGAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAA28930.2158533182318084No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT27730.20689984495568778No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27430.2046614766366576No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27390.2043630275274536No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCAAGCCATAGGTCC26910.20078163821700534No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA26900.20070702593970435No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA24220.1807109356230349No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC23680.17668187264878063No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23620.17623419898497458No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23580.17593574987577054No Hit
GTGCAGCCACAGTTCGTCTGAGATCCACTTTGGTCCCAGGGCCGAAAGTG23380.17444350432975045No Hit
GTCCCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTAGCA21780.1625055399615896No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG21730.16213247857508456No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT21530.16064023302906447No Hit
GACTGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT21440.15996872253335542No Hit
GAGTTACAGTACCAGTCTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAGACGAACTGTGGCTGCACCAT21260.15862570154193734No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20580.15355206668546895No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20530.15317900529896392No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20520.15310439302166293No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGAGCAGCACTGAACACAGA20190.15064218787072975No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA19850.14810537044249555No Hit
CAGTAATACATAGCCGTGTCGTCAGGTCTCAGGCCATTCATCTGCAGATA19800.14773230905599055No Hit
CCATAGATCAGGAGCTTAGGGGCTCTCCCTGGTCTCTGCTGATACCAATT19700.1469861862829805No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA19590.1461654512326694No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19470.14527010390505737No Hit
GTACCAGTCTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAGACGA19420.14489704251855234No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19390.14467320568664932No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG19350.1443747565774453No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19080.14236022509031818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18830.14049491815779303No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18320.13668969201544176No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18320.13668969201544176No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18210.1358689569651307No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT17930.13377981320070254No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT17790.13273524131848846No Hit
CTCCCTGGTCTCTGCTGATACCAATTTAAATAAGTGCTAATCCTCTGACTTGCCCGGCAAGTGATGGTGACT17760.13251140448658547No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCCGGACCTATGGCTTGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG17710.13213834310008044No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17500.13057148527675932No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17350.12945230111724423No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17190.12825850468042815No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTAGCACTTATTTAAATTGGTATCAGCAGAGA17170.12810928012582615No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC17160.12803466784852513No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCCAGATGACCCAGTC17060.1272885450755151No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16710.1246771153699799No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16620.12400560487427084No Hit
GAATAAGTACTATTCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAACTCCGGGAACACCCTGTAT16600.12385638031966885No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16570.12363254348776584No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC16520.1232594821012608No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16390.12228952249634772No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCAT16340.12191646110984271No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15950.1190065822951035No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15940.11893197001780248No Hit
GTTACAGTACCAGTCTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAGACGAACTGTGGCTGCACCAT15940.11893197001780248No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15920.11878274546320049No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAGATCCACTTTGGTCC15910.11870813318589947No Hit
GTGCCAGATGTGGCATCCAGATGACCCAGTCCCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA15910.11870813318589947No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA15680.11699205080797634No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT15660.11684282625337435No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15610.11646976486686932No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15560.11609670348036427No Hit
GGGCTGGAGTGGGTGGCACTTATATCAAATGATGGAAGGAATAAGTACTA15490.11557441753925725No Hit
GTATTACTGTGCGAAAGATCCGGGGCTCGTAGGTGGTCAGGGGTCCTGGC15490.11557441753925725No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15370.1146790702116452No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15280.11400755971593614No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA15250.11378372288403313No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15200.1134106614975281No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15130.11288837555642106No Hit
GAGCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG15040.112216865060712No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14970.11169457911960497No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCAGTCTATTCACTTTCGGCCC14590.10885931258216676No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14500.10818780208645773No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14310.10677016881773863No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGT14130.10542714782632051No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC14130.10542714782632051No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14090.10512869871711651No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG14010.10453180049870847No Hit
GTACTATTCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAACT13970.10423335138950444No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13970.10423335138950444No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13840.10326339178459137No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA13810.10303955495268835No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13620.10162192168396925No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGT13530.1009504111882602No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCAGTCTATTCA13460.10042812524715317No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG13430.10020428841525016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)