FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554441
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19250.1238387304503677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58600.041.966661
GGTATCA22850.040.2282751
TATCAAC67750.036.2435462
ATCAACG68300.035.9516873
GTGGTAT9350.035.9498671
TCAACGC68350.035.8250354
CAACGCA69050.035.4618575
AACGCAG70200.034.9808856
TGGTATC9200.033.921932
ACGCAGA73950.033.2251787
AGAGTAC75850.032.44754811
CAGAGTA77050.031.8976510
CGCAGAG77100.031.8738848
GCAGAGT80250.030.7102769
AGTACGG60150.029.10400813
GAGTACG60050.029.09531412
GTACGGG66450.026.13724514
TACGGGG27150.025.78995715
TATTTCG1250.001183562325.15254470
GAGTACT30750.023.44188112
Produced by FastQC (version 0.11.5)