FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669025
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26540.3966966854751317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21820.32614625761369154No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21030.3143380292216285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19600.2929636411195396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19220.28728373379171185No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18600.27801651657262433No Hit
GTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCCCTCCGCCGAAGGTG18440.2756249766451179No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17610.2632188632711782No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16000.2391539927506446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15320.2289899480587422No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13990.20911027241134486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13750.2055229625200852No Hit
GCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCAATCGGGGTCCCATCAAGGTTCAGTGGAAGTGGAT13620.20357983632898624No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTACTCTGG13510.2019356526288255No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13430.2007398826650723No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13120.19610627405552858No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCGGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA12850.19207055042786145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12560.18773588430925603No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGTAATTCTTTAAATTGGTATCAGCAGAAA12430.18579275811815701No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12290.18370016068158887No Hit
ATATTACTGTCAACAGTTTGATAATTTCCCGCTCACCTTCGGCGGAGGGA12060.18026232203579837No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCGGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA11930.17831919584469938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11800.1763760696536004No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11740.17547924218078548No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11680.17458241470797056No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11640.17398452972609393No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCTCAGGTGCCAGATGTGACAT11610.1735361159896865No Hit
ACCAGGGACAGCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCAA11450.17114457606218003No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTC11300.16890250738014276No Hit
ACATATTACTGTCAACAGTTTGATAATTTCCCGCTCACCTTCGGCGGAGGGACCAAGCTGGAGGTCAAACGAA11300.16890250738014276No Hit
AACCAGGGACAGCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCA11170.16695938118904374No Hit
TCCATGATGCGTCCGATTTGGCAATCGGGGTCCCATCAAGGTTCAGTGGA11040.16501625499794478No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCT10770.16098053137027765No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCC10720.16023317514293187No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10720.16023317514293187No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10680.15963529016105527No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9980.14917230297821457No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9830.14693023429617727No Hit
GTCCCTGGTTTCTGCTGATACCAATTTAAAGAATTACTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT9520.14229662568663354No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9320.13930720077725048No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT9150.13676618960427486No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9030.13497253465864506No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9000.1345241209222376No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8900.13302940846754605No Hit
CCTTCGGCGGAGGGACCAAGCTGGAGGTCAAACGAACTGTGGCTGCACCA8830.131983109749262No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8770.13108628227644706No Hit
CTGCTACTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTC8540.12764844363065656No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8420.1258547886850267No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8360.1249579612122118No Hit
GATTTTACTTTCACCATCCGCAACCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACAGTTTGATAATTTC8180.12226747879376704No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8170.1221180075482979No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8160.12196853630282874No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8080.12077276633907551No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7970.11912858263891483No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7930.11853069765703822No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7910.11823175516609992No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7740.11569074399312433No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7620.11389708904749449No Hit
ACCTTGATGGGACCCCGATTGCCAAATCGGACGCATCATGGATCAGAATCTTTGGGGCTGTCCCTGGTTTCTG7550.11285079032921043No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCCGCAACCTGCAGCCTGAAGATTTTGCAACATATTA7540.11270131908374126No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7380.11030977915623481No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCCGCAACCTGCAGCCTGAAGATTTTG7320.1094129516834199No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7320.1094129516834199No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7280.10881506670154328No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG7280.10881506670154328No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7260.108516124210605No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7250.10836665296513584No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7250.10836665296513584No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7010.10477934307387617No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6970.10418145809199955No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6960.10403198684653041No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6950.10388251560106124No Hit
GCAGAAACCAGGGACAGCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCAATCGGGGTCCCATCAAGGTT6870.10268674563730802No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6830.10208886065543141No Hit
GCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC6760.10104256193714735No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6740.10074361944620903No Hit
GTCCCTGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT6710.10029520570980159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAT150.00229759969.4315145
CGAGTCG200.007135839752.1522250
GTGGTAT1750.041.3566471
GTATCAA12250.038.5432661
CGTCAGA1250.035.812965
TCGTATG400.002560480534.59584840
GCATAAG400.002608930534.4638751
TATAAGA1350.033.16262416
TATCAAC14500.032.5356452
TCAACGC14550.032.4238364
ATCAACG14600.032.076943
GTGTATA1400.031.98063913
CAACGCA14750.031.7507365
GCCGTCT553.1303708E-431.5740546
CGTATGC553.1917644E-431.46967341
AACGCAG14800.031.4107956
TAACGGC553.2299274E-431.40596636
TTAACGG553.2299274E-431.40596635
TGTGTAT1550.031.10771612
TATACAC1001.4746547E-830.9919833
Produced by FastQC (version 0.11.5)