FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669025
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24950.37293075744553644No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20980.3135906729942827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18110.27069242554463585No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17410.26022943836179513No Hit
GTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCCCTCCGCCGAAGGTG17290.2584357834161653No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17170.25664212847053547No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16590.24797279623332463No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14940.2233100407309144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14730.22017114457606218No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14210.21239863981166623No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13960.20866185867493742No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13240.1978999290011584No Hit
GCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCAATCGGGGTCCCATCAAGGTTCAGTGGAAGTGGAT13090.1956578603191211No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGTAATTCTTTAAATTGGTATCAGCAGAAA12770.19087478046410822No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT12720.19012742423676246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12590.18818429804566347No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCGGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA12510.18698852808191024No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12480.18654011434550277No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12210.18250439071783564No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTACTCTGG12130.18130862075408244No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11630.1738350584806248No Hit
ATATTACTGTCAACAGTTTGATAATTTCCCGCTCACCTTCGGCGGAGGGA11580.17308770225327902No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11360.16979933485295765No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11310.1690519786256119No Hit
AACCAGGGACAGCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCA10810.16157841635215425No Hit
ACCAGGGACAGCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCAA10750.16068158887933934No Hit
ACATATTACTGTCAACAGTTTGATAATTTCCCGCTCACCTTCGGCGGAGGGACCAAGCTGGAGGTCAAACGAA10690.15978476140652442No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCGGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA10590.1582900489518329No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10500.15694480774261052No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTC10320.15425432532416578No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCT10300.15395538283322746No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10300.15395538283322746No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCTCAGGTGCCAGATGTGACAT10220.15275961286947423No Hit
GTCCCTGGTTTCTGCTGATACCAATTTAAAGAATTACTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT10210.15261014162400507No Hit
TCCATGATGCGTCCGATTTGGCAATCGGGGTCCCATCAAGGTTCAGTGGA10120.15126490041478272No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9840.14707970554164643No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9770.14603340682336236No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9700.14498710810507828No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9420.14080191323194202No Hit
CTGCTACTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTC9380.1402040282500654No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9160.13691566084974402No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT9150.13676618960427486No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9040.1351220059041142No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8690.12989051231269386No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8440.12615373117596504No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8340.1246590187212735No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8310.12421060498486604No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8190.12241695003923621No Hit
GATTTTACTTTCACCATCCGCAACCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACAGTTTGATAATTTC8070.12062329509360636No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG8070.12062329509360636No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7880.11778334142969246No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCCGCAACCTGCAGCCTGAAGATTTTGCAACATATTA7850.117334927693285No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7800.11658757146593925No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7700.11509285901124772No Hit
CCTTCGGCGGAGGGACCAAGCTGGAGGTCAAACGAACTGTGGCTGCACCA7630.11404656029296363No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7460.11150554911998804No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7360.11001083666529651No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7330.10956242292888906No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7330.10956242292888906No Hit
ACCTTGATGGGACCCCGATTGCCAAATCGGACGCATCATGGATCAGAATCTTTGGGGCTGTCCCTGGTTTCTG7310.10926348043795076No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7210.10776876798325923No Hit
CTTTGGGGCTGTCCCTGGTTTCTGCTGATACCAATTTAAAGAATTACTAA7100.10612458428309852No Hit
AGTCAGGACATTAGTAATTCTTTAAATTGGTATCAGCAGAAACCAGGGAC7070.10567617054669108No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCCGCAACC7070.10567617054669108No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCC7010.10477934307387617No Hit
GCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC7000.104629871828407No Hit
GCAGAAACCAGGGACAGCCCCAAAGATTCTGATCCATGATGCGTCCGATTTGGCAATCGGGGTCCCATCAAGGTT6930.10358357311012294No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCCGCAACCTGCAGCCTGAAGATTTTG6910.10328463061918464No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6890.10298568812824632No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6770.10119203318261649No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6720.10044467695527073No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6700.10014573446433242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG252.6078196E-455.0214043
ATAAGGC200.007452572751.5825653
GTGGTAT1850.048.3368381
GTATCAA15100.040.7711221
TATCAAC17400.035.1789132
ATCAACG17600.034.5837673
ACGGGAC1300.034.4089516
TCAACGC17800.034.3883824
AACGCAG18100.033.820936
CAACGCA18200.033.632595
CGCAGAG19400.031.743728
AGAGTAC20050.030.8885211
ACGCAGA20550.029.625837
GCAGAGT21050.029.257689
TTCGGGG2502.6557245E-1028.43816270
CAGAGTA22450.027.58640710
AGTACGG17650.026.70636713
GAGTACG17700.026.63092612
CTTACAC658.818594E-426.4525973
GGATATG3007.8216544E-1125.8528770
Produced by FastQC (version 0.11.5)