FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207384
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20940.17343281010846592No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19900.16481914618712853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18670.15463183212631607No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG18530.15347230044459756No Hit
GTATGATAATCTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17970.14883417371772362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17410.14419604699084965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16100.13334614339762663No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA15530.1286251929792013No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15090.1249809505509432No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14830.12282753457060885No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14760.1222477687297496No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14760.1222477687297496No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14410.11934893952545338No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14060.11645011032115715No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTCCCACTGGAGTTGGATCCG13390.11090092298721864No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC13340.11048680452946204No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13020.10783644639981978No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12730.10543455934483148No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12370.10245290644898392No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12160.10071360892640618No Hit
GTCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATAGTTGGTA12110.10029949046864957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27300.042.561081
GTGGTAT3650.038.7290081
TATCAAC31950.036.23182
ATCAACG32250.035.787933
TCAACGC32650.035.455014
CAACGCA33050.035.2343865
AACGCAG33600.034.6576356
AGAGTAC36200.032.17107811
CGCAGAG36650.032.058118
CAGAGTA36900.031.74753610
ACGCAGA38050.030.785477
GCAGAGT39450.029.7827579
TAACGCT605.4661924E-428.7104684
CGTATGC1103.561945E-828.30921441
CGTCGTT500.007593959627.70431943
ATGCGCG500.007662082627.65366739
TAGTACG500.007787178727.562054
AATCGAG1208.056959E-426.86474870
TATATAC906.9807193E-626.7964363
AGTACTT9450.026.61635213
Produced by FastQC (version 0.11.5)