FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207384
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19930.1650676172617825No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19670.16291420128144815No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18130.15015935278254475No Hit
GTATGATAATCTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17600.14576969713032473No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16180.13400873293003718No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16070.13309767232297262No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15970.13226943540745945No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15880.13152402218349754No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA15160.12556071639180244No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14300.11843787891838885No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14020.11611881555495186No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13490.11172915990273186No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13360.11065245191256468No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC13150.10891315438998694No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTCCCACTGGAGTTGGATCCG12920.10700820948430656No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12870.10659409102654997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12690.10510326457862619No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12650.1047719698124209No Hit
GTCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATAGTTGGTA12230.10129337476726542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12150.10063078523485486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3950.041.8192331
GTATCAA24900.039.389221
TCAACGC28450.033.984744
TATCAAC28650.033.86762
ATCAACG28750.033.7498053
CAACGCA29450.032.8307655
AACGCAG30200.031.7915126
AGAGTAC31750.030.14733311
CAGAGTA32500.029.6635110
CGCAGAG33250.029.0967528
GCAGAGT34450.028.184339
ACGCAGA34750.027.738257
AGTACTT9500.025.73253613
ACGGGTA951.0679891E-525.36816
GAGTACT9650.025.33254812
GTATTGG2100.024.5812451
AGTACGG30700.023.43990113
GAGTACG30750.023.40178512
TACTTTT10800.022.31814615
GTCCTAG1554.1967724E-822.2024151
Produced by FastQC (version 0.11.5)