FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724611
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10230.1411791982180784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.044.0648771
GTATCAA26650.040.789021
TATCAAC30650.035.0062262
TCAACGC31900.033.7419664
ATCAACG32100.033.6385233
CAACGCA31900.033.527055
AACGCAG32000.033.315156
ACGCAGA33700.031.64337
CGCAGAG34350.031.1486428
CAGAGTA35000.030.77037810
AGAGTAC34950.030.62024111
GCAGAGT36500.029.599819
GAGTACG27100.027.71890312
AGTACGG27250.027.69219613
CCGCGCA500.007766437727.57563234
GTACGGG30050.024.76945314
GTGGTAT3800.024.3647231
TACGGGA12750.024.20881715
TACGTGA901.9218204E-423.34414153
GTCTATC750.00206392622.8607251
Produced by FastQC (version 0.11.5)