FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217334
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15830.7283719988588992No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8190.3768393348486661No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7960.3662565452253214No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7480.34417072340268895No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7070.3253057505958571No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6100.28067398566262064No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA5910.27193168119116196No Hit
GTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGAT5540.25490719353621616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5330.24524464648881444No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5220.24018331232112783No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT5060.2328213717135837No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4700.21625700534660935No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT4550.20935518602703673No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4480.20613433701123615No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4380.20153312413152108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4330.1992325176916635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4240.19509142609991995No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT4210.1937110622360054TruSeq Adapter, Index 1 (95% over 21bp)
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4190.1927908196600624No Hit
GCGTAGCAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT3960.18220803003671768No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3950.18174790874874616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3750.172545482989316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3690.16978475526148695No Hit
GAGTGAAGTCTGTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATGCCA3690.16978475526148695No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTG3590.16518354238177183No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3570.16426329980582882No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT3530.1624228146539428No Hit
CACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCATCAGGGCCA3510.16150257207799976No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3470.15966208692611372No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3470.15966208692611372No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3470.15966208692611372No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTAGCAACTGG3420.15736148048625617No Hit
CTCCTGTGGGGCCAGTCAGAGCGTTAGCAGTCACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT3370.15506087404639862No Hit
GCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTAGCAACTGGCCTCTCACTTTCGGCGGCGGGA3350.15414063147045562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC3290.15137990374262655No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3150.14493820571102542No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC3080.14171735669522487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3040.13987687154333883No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTGGGGCCAGTCAGAGCGTTAGCAGTCACTTAGCCTGGTA3010.13849650767942429No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT2980.13711614381550977No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2950.13573577995159525No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC2940.13527565866362373No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT2910.1338952947997092No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2870.13205480964782315No Hit
CATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG2830.13021432449593714No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2820.12975420320796563No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2730.12561311161622205No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2710.12469286904027901No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2690.123772626464336No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG2680.12331250517636448No Hit
CATCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCA2640.12147202002447846No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT2630.12101189873650695RNA PCR Primer, Index 1 (95% over 23bp)
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG2540.11687080714476338No Hit
TCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACT2540.11687080714476338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2530.11641068585679185No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA2500.11503032199287733No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC2460.11318983684099129No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTT2410.11088923040113374No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT2380.10950886653721921No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG2370.10904874524924771No Hit
GGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAG2340.10766838138533316No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA2330.10720826009736166No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG2280.10490765365750412No Hit
GTCAGAGCGTTAGCAGTCACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG2270.10444753236953262No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2270.10444753236953262No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT2270.10444753236953262No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2270.10444753236953262No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC2260.10398741108156109No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG2250.10352728979358958No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC2240.10306716850561808No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2220.10214692592967506No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC2220.10214692592967506No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATGCCAGTGG2210.10168680464170356No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT2200.10122668335373206No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACACAGTC2190.10076656206576054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)