FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223697
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12770.10435589855985591No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12700.10378386152781285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21350.038.766631
GTGGTAT4200.032.843951
TATCAAC25450.032.3805162
TCAACGC25750.032.1371734
CAACGCA26050.031.7670735
ATCAACG25850.031.7460773
AACGCAG27100.030.5387426
ACGCAGA29150.028.2820267
TAGCGTG500.007756471627.5844045
AGAGTAC29650.027.57805311
GGTATCA14650.027.5418381
CAGAGTA30150.027.34957310
CGCAGAG30800.026.8844078
GTATTAG2100.026.2751581
GCTATAC809.5991316E-525.860383
GCAGAGT32100.025.795639
GAGTACG28050.023.73910712
AGTACGG28300.023.52939813
TTCGGGG6250.022.57505870
CGTATAG800.002863147321.6094837
Produced by FastQC (version 0.11.5)