FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540599
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCAGGTCTCAGGCTGTTCATTTGCAGATACA14820.27414035172096135No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8240.15242351539680984No Hit
GTGTATTACTGTGCGAAAGATTATAGTGGCTACGAGTTCGTTCTTTTGTC7270.13448045593869024No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7030.13004093607276374No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7020.12985595607835013No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGTCATGACTAC6690.12375161626270118No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6540.12097691634649713No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA6470.11968205638560189No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6410.11857217641912028No Hit
GAGTTACAGTCCCCCTCGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAACCGAACTGTGGCTGCACCAT6400.11838719642470667No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5780.1069184367710632No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5740.10617851679340878No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5600.10358879687161833No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5590.10340381687720472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5490.10155401693306869No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5480.1013690369386551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7800.038.1212161
CTAGTCA3250.036.3338370
ATCAACG8650.034.3561253
TCAACGC8600.034.1540574
CAACGCA8550.033.9496275
TATCAAC9000.033.0200542
AACGCAG9000.032.63616
GTCGTAA450.00449150930.84471548
GTATAAG1151.6607373E-930.0652681
AGAGTAC10100.028.73953411
TGTATAA854.36711E-628.45777514
CGCAGAG10400.028.2427778
CTTAGAA500.00765324827.65748629
ACGCAGA10900.026.9472357
GTGTATA906.8110967E-626.87678713
GGTATCA5800.026.8254781
CGTAAAC658.340852E-426.70494550
GCAGAGT11150.026.0331219
CCCTAGT3850.025.9322668
GTGCTAT809.4125455E-525.9312931
Produced by FastQC (version 0.11.5)