FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489178
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17360.35488104534545706No Hit
GCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTACTCCTATCCCACAC14320.29273597749694386No Hit
CCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG13550.2769952859695244No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13520.2763820122736509No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT13160.26902272792316906No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12780.2612545944421049No Hit
GACCTAGGACGGTCATCTTGGTCCCTCCGCCGAAAATTATAAGATCACTA11670.23856346769478595No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11450.2340661272583804No Hit
CTCCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC10460.21382809529455535No Hit
GGATTACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC10310.21076172681518793No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9970.2038112915952884No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9430.1927723650695657No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCATCTTGGTCCCTC9140.18684405267612197No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8990.18377768419675455No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8850.1809157402826783No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8690.1776449472380197No Hit
GGATAGGAGTAGTGATCTTATAATTTTCGGCGGAGGGACCAAGATGACCGTCCTAGGTCAGCCCAAGGCTGCCCC8300.16967238919166439No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGTAACAACATTGGAAGT8080.16517504875525882No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7960.1627219539717649No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7960.1627219539717649No Hit
CCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCT7950.1625175294064737No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7850.1604732837535621No Hit
GTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG7540.15413612222953607No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7330.14984320635842166No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7190.14698126244434542No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7070.14452816766085147No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7040.14391489396497797No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6970.14248392200793986No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGT6710.13716888331036964No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6560.13410251483100222No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT6560.13410251483100222No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6540.13369366570041988No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6460.13205826917809058No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6080.12429013569702643No Hit
GGGTGGCCGTGTTCCCAGAATTGGAGCCAGACAATCGCTCAGGGATCCCTGAGGGCCGTTTACTATCCTCATAGA6050.12367686200115297No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6010.12285916373998831No Hit
GGCTCTGTGACCTCCTATGTCCTGAGTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC5960.1218370409135325No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAATTGGAGCCAGA5950.12163261634824134No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5930.12122376721765901No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5920.12101934265236786No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA5890.12040606895649436No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC5850.11958837069532971No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5760.11774854960770927No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5700.11652200221596229No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGC5630.11509103025892417No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5610.11468218112834185No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG5610.11468218112834185No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG5590.11427333199775952No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5570.1138644828671772No Hit
GTATATTACTGTGCGAAACAGACGGACGTGGGAGATTACAATTTTTGGAGTGGTTATCCGGGGCACTTGGATTA5520.11284236004072137No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5490.11222908634484789No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGAGTCAGCCAC5450.11141138808368324No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5390.11018484069193626No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGG5360.1095715669960628No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT5270.10773174590844233No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA5220.10670962308198652No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5190.10609634938611302No Hit
GTGTTGGTCGTCTATGAGGATAGTAAACGGCCCTCAGGGATCCCTGAGCG5180.10589192482082187No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT5160.10548307569023954TruSeq Adapter, Index 1 (95% over 21bp)
TCTATGAGGATAGTAAACGGCCCTCAGGGATCCCTGAGCGATTGTCTGGCTCCAATTCTGGGAACACGGCCACC5140.10507422655965722No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT5040.1030299809067456No Hit
CTATTACTGTCAGGTGTGGGATAGGAGTAGTGATCTTATAATTTTCGGCG4980.10180343351499863No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC4940.10098573525383397No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT4900.10016803699266932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC2600.059.5082633
TTATACA2550.056.630082
CTTATAC2700.056.0709421
CGATGTT350.0055379755.59437670
CGTTGCG200.00668344453.02088564
GCGTCGC200.00740827151.65831432
AACCGCG200.007456375851.5738267
GTATCAA8350.045.7388731
GGTATCA2650.045.4434361
TATCAAC8800.042.9781882
AGCGTCA405.099378E-542.9781883
TCAACGC9750.039.1432154
TACACAT3700.039.028845
CAACGCA10050.037.974765
ATCAACG10050.037.974763
TCGTATG3750.037.8002440
TACGGGT1100.037.5082415
ATACACA3850.037.5082364
TATGCCG3700.037.44591543
AACGCAG10350.036.8740436
Produced by FastQC (version 0.11.5)