FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683861
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25980.37990176366249867No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22820.3336935429860746No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT22620.3307689720571871No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21080.30824977590475255No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20900.30561766206875374No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17640.2579471559278859No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16790.24551772948011363No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16450.24054595890100472No Hit
GCCTTGGGCTGACCTTTGACGGTCAGCCTGGTCCCTCCGCCGAACACCCA16320.2386449877972278No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16070.2349892741361183No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15830.2314797890214532No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15320.22402213315278982No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14780.21612579164479329No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC14290.2089605928690187No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14000.2047199650221317No Hit
AGCCAGGACAGGCCCCTCTACTTGTCGTCTATGGTAAGAACAACCGGCCC13890.2031114510112435No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13520.19770099479280148No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13400.19594625223546888No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12900.1886348249132499No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12830.18761122508813927No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT12360.1807384834052534No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTGCATAAAAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT12320.1801535692194759No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG12160.17781391247636583No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12010.17562048427970012No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11520.1684552855039255No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11450.16743168567881483No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA11420.1669930000394817No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11410.1668467714930373No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11190.16362974347126097No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11140.16289860073903908No Hit
CCATTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCAAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT11120.1626061436461503No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10640.15558717341682007No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA10590.1548560306845982No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10430.1525163739414881No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10240.1497380315590449No Hit
GTAGAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGTTTGCATAAAAGCTT10140.1482757460946011No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10110.14783706045526795No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT9990.14608231789793538No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9690.14169546150460402No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGATGTTCCCCTGCTGCCACCTGCTCTTGTCCACGGTGAG9380.13716237656482824No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9370.13701614801838385No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9150.13379911999660749No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9040.13219060598571933No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9030.13204437743927494No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8900.130143406335498No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATTGGGTGTTCGGCG8720.12751129249949916No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTTGTGTCTGTGGCCTTGGGA8660.1266339212208329No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG8490.12414803593127843No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG8370.12239329337394589No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8210.1200536366308358No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8170.11946872244505827No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8140.11903003680572513No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC8070.11800643698061448No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTTGACGGTCAGCCTGGTCCCTC8070.11800643698061448No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA7960.1163979229697263No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7900.11552055169106003No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG7850.11478940895883812No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTT7820.11435072331950498No Hit
GCCTGGTCCCTCCGCCGAACACCCAATGGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTT7560.11054878111195113No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7520.10996386692617359No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA7490.10952518128684045No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA7430.10864781000817418No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7430.10864781000817418No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7400.10820912436884104No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT7340.10733175309017476No Hit
ATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA7310.10689306745084162No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG7250.10601569617217535No Hit
GGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCC7160.10469963925417591No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATTGGGTGTTCGG7060.10323735378973212No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7060.10323735378973212No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7020.1026524396039546No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7010.10250621105751022No Hit
GTGGTAACCATTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCAAAGGTCAGCCCAAGGCTGCCCCCTCGGTCA7010.10250621105751022No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAG6950.10162883977884395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3850.058.0905881
AGGGGGG3200.055.8908370
TTATACA4000.055.0036962
TATACAC4050.054.324643
GTTAGTC200.00644982253.50233566
GTGGTAT1500.052.7581671
TACACAT4850.046.072695
TCGTATG4900.044.39415740
ATACACA5200.044.2938424
ATCTCGT4450.044.16949537
CGTATGC4850.044.1593541
TCTCGTA4550.043.96951338
GTATCAA14700.043.7700841
ATCTCCG5050.043.57047310
TGGTATC1900.043.4239732
TCTACAT4600.043.3992532
ACGAGAC5050.042.88968322
CTCGTAT5050.042.36688639
GCCGTCT5000.042.28037346
ATGCCGT5000.042.2274644
Produced by FastQC (version 0.11.5)