FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683861
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49850.7289493040252332No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25960.3796093065696099No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19940.29157972161009327No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18980.2775417811514328No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18430.26949921109699193No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18330.26803692563254816No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17450.2551688135454427No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16280.23806007361145026No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15100.22080510513101345No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14720.215248420366127No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14460.21144647815857315No Hit
AGCCAGGACAGGCCCCTCTACTTGTCGTCTATGGTAAGAACAACCGGCCC13540.19799345188569023No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13300.1944839667710251No Hit
GCCTTGGGCTGACCTTTGACGGTCAGCCTGGTCCCTCCGCCGAACACCCA13220.19331413839947006No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13100.19155939584213752No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13010.19024333892413808No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC13000.19009711037769372No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12580.18395551142702976No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12400.18132339759103092No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12220.1786912837550321No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12200.17839882666214332No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTGCATAAAAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT11980.17518179864036698No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT11550.16889397114325863No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11470.1677241427717036No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG11070.16187500091392842No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11000.16085140108881776No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10810.15807305870637456No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10710.15661077324193073No Hit
CCATTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCAAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT10530.15397865940593192No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA10050.14695968917660168No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9870.14432757534060284No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA9850.1440351182477141No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9830.1437426611548253No Hit
GTAGAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGTTTGCATAAAAGCTT9750.1425728327832703No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9710.14198791859749277No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9600.14037940458660458No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9380.13716237656482824No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGATGTTCCCCTGCTGCCACCTGCTCTTGTCCACGGTGAG9350.1367236909254951No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9280.13570009110038445No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9250.1352614054610513No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTTGTGTCTGTGGCCTTGGGA9240.13511517691460692No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9180.13423780563594062No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT9150.13379911999660749No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8830.12911980651038735No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8740.12780374959238794No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT8570.12531786430283348No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC8400.12283197901327902No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8210.1200536366308358No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7910.11566678023750442No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT7890.11537432314461565No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7770.1136195805872831No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG7680.11230352366928366No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7670.11215729512283928No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7650.11186483802995054No Hit
GTGGTAACCATTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCAAAGGTCAGCCCAAGGCTGCCCCCTCGGTCA7650.11186483802995054No Hit
GCCTGGTCCCTCCGCCGAACACCCAATGGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTT7630.11157238093706177No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATTGGGTGTTCGGCG7580.11084123820483988No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7520.10996386692617359No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTTGACGGTCAGCCTGGTCCCTC7510.10981763837972922No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG7340.10733175309017476No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7250.10601569617217535No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7190.10513832489350905No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGTT7170.1048458678006203No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATTGGGTGTTCGG7140.10440718216128717No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7130.10426095361484279No Hit
ATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA7130.10426095361484279No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7000.10235998251106584No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6920.10119015413951081No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAG6860.10031278286084452No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA6850.10016655431440015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1800.059.1324652
GTGGTAT2400.047.2244071
TAACATA306.418708E-445.840327
TGTATAA405.1344938E-542.93128217
GTATCAA14500.042.161481
GGTATCA5500.039.965121
TATCAAC16250.037.610012
ATCAACG17100.035.7405053
TCAACGC17200.035.133474
GTCTCGC701.0077165E-634.3450241
CAACGCA17700.034.334985
AACGCAG18050.033.859426
CGCAGAG18550.032.5980348
AGAGTAC18700.031.96909111
CAGAGTA18950.031.54733710
GACAGGT1208.185452E-1131.4829371
GTGTAAC553.3427478E-431.2227481
ACGGGTA1202.8103386E-928.62294416
GTATAAG605.566988E-428.61875718
TACGGGT6550.028.3253415
Produced by FastQC (version 0.11.5)