FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687211
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTCAGGTCTCAGGCTGCTCATTTCCATATACA69830.41387828789641606No Hit
GTGTACTACTGTGCGAGAGATGAGGGGTACGGTGACTACGACGTTGTCGT63660.37730906211493404No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61340.36355855906581924No Hit
CTACAAACTCCATCCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCAT47390.2808777325420472No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42850.2539694205407622No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40910.24247115506003694No Hit
GAGTTTGTAGAGCTCGCATGCAGTAATAAATGCCAACATCCTCAGCCTCCACTCTGCTAATTGTTAGTGTAAAAT37990.22516448742925457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37540.22249736399300385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37510.22231955576392046No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37370.22148978402819802No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35860.21254010316433453No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC34990.20738366452091647No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33610.19920448598308096No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33510.19861179188613637No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC33470.1983747142473585No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA33200.1967744401856081No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32990.19552978258202441No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32500.19262558150699585No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG32370.1918550791809679No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31610.1873506040441889No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA31570.18711352640541104No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31420.18622448525999416No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAAC30960.18349809241404896No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT30750.18225343481046533No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30550.18106804661657613No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30530.1809495077971872No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT29800.1766228408894916No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG28510.1689770870389062No Hit
GTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTTCTGATCTATTTGGGTT28140.1667841188802112No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26800.15884201798115352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26420.15658978041276403No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26360.15623416395459727No Hit
CTCCTACATAGTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTTCTGAT25940.15374484874742994No Hit
GTCAGGGACCCCGGAGGCCCGCTTAGAACCCAAATAGATCAGAAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA25740.15255946055354072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25000.14817352423615066No Hit
CGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTCATTTCATATGATGGAAGCA24860.14734375250042822No Hit
AGCCAGGGCAGTCTCCACAACTTCTGATCTATTTGGGTTCTAAGCGGGCC24740.1466325195840947No Hit
CTCCTGTGCAGCCTCTAGATTCACCTTCAGAGGTCACGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG24640.1460398254871501No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCGTGACCTC24330.14420247378662182No Hit
CAATTAGCAGAGTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCGA24160.143194893821816No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA23760.14082411743403758No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG23490.13922384337228716No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACT23160.13726795285236998No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22940.13596402583909184No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22820.13525279292275832No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC22510.13341544122223006No Hit
ACATAGTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC22390.13270420830589655No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22330.1323485918477298No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGTTGACTCAGTCTCCACT22260.13193370597986856No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAACAATTAGCAGAG22240.13181516716047964No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22090.13092612601506273No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT21970.1302148930987292No Hit
GCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC21930.12997781545995138No Hit
GTATTGAGTGCTTCCATCATATGAAATGACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG21750.12891096608545108No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGTTGA21530.12760703907217294No Hit
GGGCTGGAGTGGGTGGCAGTCATTTCATATGATGGAAGCACTCAATACTA21450.12713288379461726No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21250.12594749560072807No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21160.12541407091347792No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20730.12286548629661612No Hit
CAGTAATAAATGCCAACATCCTCAGCCTCCACTCTGCTAATTGTTAGTGTAAAATCTGTGCCTGATCCACTGCCA20700.12268767806753275No Hit
CAGTAGTACACAGCCGTGTCCTCAGGTCTCAGGCTGCTCATTTCCATATA20680.12256913924814383No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTTCTGATCTATTTGGGTTCTAAGCGGGCCTCCGGGGTCCCTGA20610.12215425338028262No Hit
GCCCAGCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAAC20340.12055397931853218No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20290.12025763227005988No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20080.11901297466647622No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19990.11847954997922608No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGTTGACTCAGTCTCCACTCTCC19970.11836101115983715No Hit
TTATTACTGCATGCGAGCTCTACAAACTCCATCCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG19900.11794612529197593No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19750.11705708414655902No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19720.11687927591747564No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA19680.1166421982786978No Hit
GTCGTGTCCCTTGGCCGAAGGATGGAGTTTGTAGAGCTCGCATGCAGTAATAAATGCCAACATCCTCAGCCTCCA19540.11581242654297537No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19540.11581242654297537No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19430.1151604630363363No Hit
GATTTTACACTAACAATTAGCAGAGTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCGAGCTCTACAAACT19350.11468630775878061No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA19270.11421215248122493No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGAT19260.11415288307153046No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19210.11385653602305817No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19050.11290822546794681No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAG19030.11278968664855787No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19020.11273041723886341No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18910.11207845373222436No Hit
CTTCTACTACTACGGTTTGGACGTCTGGGGCCAAGGAACCACGGTCACCG18790.11136722081589083No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG18160.10763324800513985No Hit
GTACTACTGTGCGAGAGATGAGGGGTACGGTGACTACGACGTTGTCGTCGATCACTTCTACTACTACGGTTTGGA18140.10751470918575093No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18140.10751470918575093No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTTCTGATCTATTTGGGTTCTAAGCGGGC18120.107396170366362No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17930.10627005158216726No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17820.1056180880755282No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17510.10378073637499993No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17500.10372146696530546No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17350.10283242581988856No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG17210.10200265408416612No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC17010.1008172658902769No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC17000.10075799648058247No Hit
ATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGT16950.10046164943211015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.045.7871741
GTGGTAT3950.045.398341
TGGTATC4250.042.1699942
TCTGGAG24700.040.73742370
TTGTACG350.001354573239.3895573
GTATCAA28700.039.051271
TATCAAC31800.035.2245452
GGTTTGG25000.033.54047870
TGTATAA1350.033.1903314
ATCAACG33950.032.892313
TCAACGC34100.032.747624
CAACGCA34750.032.2342575
AACGCAG34800.032.1879466
CGCAGAG36550.030.64688
CAGAGTA37150.030.245510
ATGTGTA1600.030.15852211
GCTTGTA1151.713488E-929.98721
AGAGTAC37650.029.66074611
CTCTGGA25700.028.91189669
AGTGGTG5750.028.7715025
Produced by FastQC (version 0.11.5)