FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500943
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70120.4671729705924875No Hit
CTCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCGAAGCTCCTCAT59610.3971503248291241No Hit
GAGTAGTGGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT49430.3293262968680356No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46790.31173735444983586No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44040.2934155394308778No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42440.28275557432893855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39250.26150226890694717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38250.25483979071823515No Hit
TCTCAGGGGTCCCCTCGAGGTTTAGTGGCAGTGGATCTGGGACAGATTTC37340.2487769355665072No Hit
ACCTACTACTGTCATCAGAGTAGTGGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA36180.24104846086760123No Hit
CCACTACTCTGATGACAGTAGTAGGTTGCAGCATCTTCAACTTCCAGGCCATTGATGGTGAGGGTGAAATCTGTC35440.23611822700795432No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC34950.23285361269548546No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34830.23205411531283998No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34710.23125461793019456No Hit
GTGTAAGAGATTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT34530.23005537185622638No Hit
GTAGTAGGTTGCAGCATCTTCAACTTCCAGGCCATTGATGGTGAGGGTGA34070.22699063188941884No Hit
GATTTCACCCTCACCATCAATGGCCTGGAAGTTGAAGATGCTGCAACCTACTACTGTCATCAGAGTAGTGGTTTC33540.22345951844940148No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA33530.2233928936675144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32530.2167304154788023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28330.1887480070862118No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28210.18794850970356636No Hit
TCCCAGCCCTTCTCAGGGGTCCCCTCGAGGTTTAGTGGCAGTGGATCTGG27320.18201890411561264No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27310.18195227933372554No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26350.17555630027256197No Hit
CTACTACTGTCATCAGAGTAGTGGTTTCCCGTGGACGTTCGGCCAAGGGA24860.16562920777138107No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24770.16502958473439697No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24660.16429671213363864No Hit
CCCCTGAGAAGGGCTGGGAAGCATAGTTGATGAGGAGCTTCGGAGACTGA24340.1621647191132508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24250.16156509607626673No Hit
CAGTAGTAGGTTGCAGCATCTTCAACTTCCAGGCCATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA24190.16116534738494398No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23950.15956635261965313No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG23680.15776748350870087No Hit
GTCTCCGGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT23430.15610186396152287No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTCCTGACTCAGTCTCCGGACTTT23030.15343687268603803No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22750.15157137879319868No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22530.15010563359168203No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22320.1487065131720525No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22290.14850663882639115No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21300.14191078541956623No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21250.14157766151013063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20990.1398454171810655No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20420.13604780461349963No Hit
GTTTAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGCCTGGAAGTTGAAGATGCTGCAACCTA20280.13511505766707996No Hit
GGGCTGGGAAGCATAGTTGATGAGGAGCTTCGGAGACTGATCTGGTTTCT20000.1332495637742406No Hit
AAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTT19340.12885232816969064No Hit
GTCCTGACTCAGTCTCCGGACTTTCAGTCTGTGACTCCAAAGGAGAAAGT19100.12725333340439976No Hit
AGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCT18730.12478821647457632No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18460.12298934736362406No Hit
CACCATCAATGGCCTGGAAGTTGAAGATGCTGCAACCTACTACTGTCATCAGAGTAGTGGTTTCCCGTGGACGTT18410.12265622345418847No Hit
GCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTCCTGACTCAGTCTCCGG18170.12105722868889757No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17770.11839223741341277No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17680.11779261437642868No Hit
CCTCATCAACTATGCTTCCCAGCCCTTCTCAGGGGTCCCCTCGAGGTTTAGTGGCAGTGGATCTGGGACAGATTT17630.1174594904669931No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17590.1171929913394446No Hit
GAGCATTGGTAATCTCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCGAAGCTCCTCATCAACTATGCTTC17480.1164601187386863No Hit
CTGCTGCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTCCTGACTCAGTC17020.11339537877187873No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16820.11206288313413634No Hit
GTCTCCGAAGCTCCTCATCAACTATGCTTCCCAGCCCTTCTCAGGGGTCCCCTCGAGGTTTAGTGGCAGTGGAT16380.10913139273110305No Hit
GTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGCCGGGCCAGTCAGAGCATTGGTAATCTCTTACACTGGTA16240.10819864578468337No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15860.10566690407297279No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTCGAGGGGACCCCT15760.1050006562541016No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15450.10293528801560085No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15410.10266878888805238No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15350.10226904019672967No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15140.10086991977710012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.050.993111
CGTACGA305.791582E-446.81024659
GTATCAA42950.041.1479071
GACCGAT1400.036.8601626
TATCAAC49400.035.6159742
GCGTACG400.002397023835.0671158
ATCAACG50750.034.600843
TCAACGC50750.034.600844
ATCGCGT400.002588273534.5225637
CAACGCA51600.034.097465
GTGGTAT4150.033.9690631
AACGCAG52150.033.8037456
ACGCAGA54800.032.294497
CGACTCG450.00390716731.73938866
CGCAGAG56900.031.2837878
AGAGTAC56600.031.26745811
ACCGATC1550.031.07765627
TGGTATC4550.030.9652292
TATACAC2250.030.5456433
GCAGAGT59200.030.1264139
Produced by FastQC (version 0.11.5)