FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500943
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68000.453048516832418No Hit
CTCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCGAAGCTCCTCAT60460.4028134312895293No Hit
GAGTAGTGGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT49250.32812705079406745No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42020.2799573334896795No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41520.2766260943953235No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41320.2752935987575811No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40090.2670987505854652No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37540.2501094312042496No Hit
ACCTACTACTGTCATCAGAGTAGTGGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA36840.24544569647215117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35150.23418610833322784No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC34910.23258711356793693No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA33970.22632438407054764No Hit
TCTCAGGGGTCCCCTCGAGGTTTAGTGGCAGTGGATCTGGGACAGATTTC33240.22146077499278788No Hit
GTGTAAGAGATTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT33070.22032815370070682No Hit
CCACTACTCTGATGACAGTAGTAGGTTGCAGCATCTTCAACTTCCAGGCCATTGATGGTGAGGGTGAAATCTGTC32480.21639729156936674No Hit
GTAGTAGGTTGCAGCATCTTCAACTTCCAGGCCATTGATGGTGAGGGTGA32150.21419867376709176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31420.20933506468933197No Hit
GATTTCACCCTCACCATCAATGGCCTGGAAGTTGAAGATGCTGCAACCTACTACTGTCATCAGAGTAGTGGTTTC29600.19720935438587608No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29330.19541048527492383No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27730.1847505201729846No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27080.18041990935032176No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25020.16669520428157497No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24920.16602895646270377No Hit
TCCCAGCCCTTCTCAGGGGTCCCCTCGAGGTTTAGTGGCAGTGGATCTGG24810.16529608386194547No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24740.16482971038873562No Hit
CTACTACTGTCATCAGAGTAGTGGTTTCCCGTGGACGTTCGGCCAAGGGA24710.16462983604307427No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23750.1582338569819107No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG23690.157834108290588No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTCCTGACTCAGTCTCCGGACTTT23590.15716786047171677No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22700.15123825488376308No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22650.15090513097432748No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22360.14897301229960097No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22350.14890638751771385No Hit
CCCCTGAGAAGGGCTGGGAAGCATAGTTGATGAGGAGCTTCGGAGACTGA22090.14717414318864874No Hit
CAGTAGTAGGTTGCAGCATCTTCAACTTCCAGGCCATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA21000.13991204196295262No Hit
GTCTCCGGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT20930.1394456684897428No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20680.1377800489425648No Hit
GTCCTGACTCAGTCTCCGGACTTTCAGTCTGTGACTCCAAAGGAGAAAGT20640.1375135498150163No Hit
GGGCTGGGAAGCATAGTTGATGAGGAGCTTCGGAGACTGATCTGGTTTCT20500.1365808028685966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20410.13598117983161254No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20380.13578130548595116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19880.13245006639159515No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19830.13211694248215955No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19430.12945195120667474No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTAAACCTCGAGGGGACCCCT19370.12905220251535202No Hit
GTTTAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATGGCCTGGAAGTTGAAGATGCTGCAACCTA18590.12385546952815663No Hit
AAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTT18440.12285609779984982No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18240.12152360216210742No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17950.11959148348738094No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17870.11905848523228396No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17870.11905848523228396No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17750.11825898784963852No Hit
CTGCTGCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTCCTGACTCAGTC17310.11532749744660523No Hit
CACCATCAATGGCCTGGAAGTTGAAGATGCTGCAACCTACTACTGTCATCAGAGTAGTGGTTTCCCGTGGACGTT16910.11266250617112043No Hit
AGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCT16740.11152988487903936No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16700.11126338575149089No Hit
CCTCATCAACTATGCTTCCCAGCCCTTCTCAGGGGTCCCCTCGAGGTTTAGTGGCAGTGGATCTGGGACAGATTT16610.11066376271450683No Hit
GCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTCCTGACTCAGTCTCCGG16540.11019738924129698No Hit
GAGCATTGGTAATCTCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCGAAGCTCCTCATCAACTATGCTTC16300.10859839447600608No Hit
GGGTTTCTGCTGCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTCCTGAC16250.10826527056657048No Hit
GTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGCCGGGCCAGTCAGAGCATTGGTAATCTCTTACACTGGTA15930.10613327754618264No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15450.10293528801560085No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15170.10106979412276149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)